TCSPC for FLIM and FRET in - Boston Electronics Corporation

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SPC-144Photon ChannelsPrincipleConstant Fraction Discriminator (CFD)Time Resolution (FWHM / RMS, electr.)8 ps / 5 psOpt. Input Voltage Range- 50 mV to - 1 VMin. Input Pulse Width400 psLower Threshold- 20 mV to - 500 mVUpper Threshold -Zero Cross Adjust- 100 mV to + 100 mVSynchronisation ChannelsPrincipleConstant Fraction Discriminator (CFD)Opt. Input Voltage Range- 50 mV to - 1 VMin. Input Pulse Width400 psThreshold- 20 mV to -500 mVFrequency Range0 to 200 MHzFrequency Divider 1-2-4Zero Cross Adjust-100 mV to + 100 mVTime-to-Amplitude Converters / ADCsPrincipleRamp Generator / Biased AmplifierTAC Range50 ns to 2 usBiased Amplifier Gain 1 to 15Biased Amplifier Offset0 to 100% of TAC RangeTime Range incl. Biased Amplifier3.3 ns to 2 usmin. Time / Channel813 fsADC Principle50 ns Flash ADC with Error CorrectionDiff. Nonlinearity< 0.5% rms, typ.
The TCSPC Imaging PackageSPC-154Four-Channel Time-Correlated Single Photon Counting ModuleFour fully parallel TCSPC channelsPicosecond resolutionUltra-high sensitivityMulti-detector capability in all four channelsHigh-speed on-board data acquisitionPhoton distribution and time-tag modesImage acquisition by synchronisation with ext. scannerUnlimited sequential recording of curves or imagesImaging in histogram mode and in time-tag modeWorks at any scan rate of CLSMs or MPLSMsTime channel width down to 813 fsElectrical time resolution down to 8 ps fwhm / 4 ps rmsReversed start/stop: Laser repetition rates up to 150 MHzTotal saturated count rate 40 MHzTotal useful recorded count rate up to 20 MHzChannel dead time 100 nsMulti-wavelength FLIMDouble-exponential FLIMFast-Acquisition FLIMFast Sequential FLIMSingle and double-exponential FRET imagingFCS, FCCS, FIDA, FILDA, BIFLFCS ImagingDOT, static and dynamic brain imagingTransient fluorescence lifetime effectsBecker & Hickl GmbHNahmitzer Damm 3012277 Berlin, BerlinTel. +49 / 30 / 787 56 32Fax. +49 / 30 / 787 57 34email: info@becker-hickl.comwww becker-hickl comUS Representative:Boston Electronics Corptcspc@boselec.comwww boselec.comUK Representative:Photonic Solutions PLCsales@psplc comwww psplc comCovered by patents DE 43 39 784 and DE 43 39 787
Page 1 and 2: Boston Electronics Corporation91 Bo
Page 3 and 4: FLIM Upgrade Kits for Confocal Micr
Page 5 and 6: ContentsIntroduction...............
Page 7 and 8: concentration, fig. 2.Fluorescence
Page 9 and 10: Microscopy TechniquesWide-Field Flu
Page 11 and 12: fluorescence lifetime of the sample
Page 13 and 14: ns or less. Due to the high gain an
Page 15 and 16: Second generation image intensifier
Page 17 and 18: Appreciable loss of photons can occ
Page 19 and 20: Standard gated image intensifier de
Page 21 and 22: If a frequency between 100 MHz and
Page 23 and 24: fraction of unmodulated light is de
Page 25 and 26: intensifiers. The dielectric losses
Page 27 and 28: Fortunately, decay curves are eithe
Page 29 and 30: Time-Correlated Single Photon Count
Page 31 and 32: Zeissb&hSPC-730 TCSPC Imaging Modul
Page 33 and 34: R5900L16DiscriminatorsEncoderDetect
Page 35 and 36: Features of the TCSPC imaging techn
Page 37 and 38: containing CFP and YFP measured by
Page 39 and 40: CathodeCathodeMultichannelPlateMult
Page 41 and 42: FCS / lifetime data in selected spo
Page 43 and 44: [44]G. J. Brakenhoff, M. Müller, R
Page 45 and 46: Time-Correlated Single Photon Count
Page 47 and 48: The TCSPC Microscopy SolutionSPC-83
Page 49: The TCSPC Imaging PackageSPC-144Fou
Page 53 and 54: Multi-Spectral FLIM SystemMS-FLIMFl
Page 55 and 56: Lifetime Upgrade Kit for Zeiss LSM-
Page 57 and 58: FLIM Upgrade Kits for Olympus FV100
Page 59 and 60: DCS-120Confocal Scanning SystemSyst

TCSPC for FLIM and FRET in - Boston Electronics Corporation

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