IERG Abstracrt Book.indd - LV Prasad Eye Institute

IPT 012Oral Presentation37Animal Models of Ocular Fibrosis: Approaches for Therapeutic PreventionSarbani Hazra, Himangshu Palui, Aditya Konar, Debiprasad Jana, Geeta K Vemuganti, Ruchi MittalDept of Veterinary Surgery & Radiology, West Bengal University of Animal and Fishery Sciences,Kolkata.Purpose: Fibrosis is a phenomenon associated with wound healing; in the eye too fibrosis isassociated with different healing processes. In the eye the fibrosis may have severe outcome i.e.visual impairment and blindness some ocular fibrotic conditions which lead to blindness are,corneal ulceration, anterior sub capsular and complete cataract, posterior capsular opacification,pterygium, PVR and retinal pigment epithelial (RPE). In order to understand the pathobiology ofTGF beta or any other cytokine on fibrotic conditions appropriate animal models are required.The objective of this study is to create animal models of the fibrotic eye diseases, establish EMTas a phenomenon underlining the pathology, to study the effect of some antifibrotic agents inthe animal models.Methods: The animal models of corneal ulceration, anterior subcapsular, complete cataract,and posterior capsular opacification were created in White New Zealand rabbits. Fibroticchanges and EMT were studied by clinical evaluation, histology of the anterior and posteriorcapsule, immunohistochemistry of the histological sections with PCNA and immunoblottingwith PCNA and alpha-SMA.Results: Models of anterior subcapsular cataract, complete cataract and posterior capsularopacification corneal ulceration, were established clinically. The histological, immunoblottingfindings supported the clinical findings. PCO was established by presence of myofibroblasts inthe posterior capsule.Conclusions: The models of anterior subcapsular cataract, complete cataract and posteriorcapsular opacification can be used for the studies on pathogenesis of the condition .The modelscan potentially be used for therapeutic drug trials for studies on prevention of ocular fibrosis.Paper Session III, Community Eye Health, July 1, 2010, 14.30-16.00 hrsChairs: Vinay Nangia and Ajit B MajjiIPT 013Indian Vision Function Questionnaire: Re-Evaluating Using Rasch AnalysisVijaya K Gothwal, Deepak K Bagga, Rebecca SumaliniMeera and L B Deshpande Centre, L V Prasad Eye Institute, Hyderabad, India.Purpose: Previous psychometric evaluation of the Indian Vision Function Questionnaire (IND-VFQ) focused on classic assessments of reliability and validity. Our aim was to investigate thepsychometric properties of the IND-VFQ using the Rasch measurement model and if flawed,to revise the IND-VFQ creating valid measurement scales.Methods: The 45-item IND-VFQ was administered in a face-to-face interview to 236 visually