IERG Abstracrt Book.indd - LV Prasad Eye Institute

68 Basic Poster SessionsMethods: 20 Clinical isolates with Fusarium ocular infections were retrieved from storage.Morphologic classification was determined. DNA was extracted and purified, and the InternalTranscribed Spacer (ITS) region was amplified and sequenced. The antifungal susceptibilitiesof the Fusarium species from clinical isolates were tested with two different antifungal drugsVoriconazole and Natamycin, according to the National Committee for Clinical LaboratoryStandards (NCCLS).The ATCC strains of Aspergillus flavus, Aspergillus fumigatus, Candidaparapsilosis were used as quality control.Results: The morphological observation showed up to genus level as Fusarium species. Fromthe 20 samples of the clinical isolates, 14 were Fusarium solani and other 6 were Fusarium solanicomplex (Nectria haematococca) using ITS primers (1 & 4), which amplifies 575 bp sequencefragment. The antifungal susceptibility (MIC) of clinical isolates was found to be 2µg/ml to 8µg/ml. The antifungal susceptibility of the two different drugs (voriconazole & Natamycin) showssimilar range of MIC.Conclusions: ITS region are highly conserved and multi copy gene. Hence, sequence variationwithin the ITS may be useful for the species identification. Fusarium species tends to be sensitiveto these antifungal drugs.IBP 014Association of G>A Substitution in Intron 4 of Indoleamine 2,3 dioxygenase (IDO)gene with Age Related CataractM Mamata, 1 G Sridhar, 2 K Ravi Kumar Reddy, 3 T Naga Raju, 2 T Padma 11Department of Genetics, O U, Hyderabad, 2 Department of Zooogy, O U, Hyderabad,3Sarojini Devi Eye Hospital, Hyderabad, IndiaPurpose: IDO is the first rate limiting enzyme in Tryptophan metabolism which catalyzesoxidative degradation of Tryptophan in kynurenine path way. Variations in IDO gene areimplicated in cataract development. Hence we attempted to screen for the mutation in patientsof Age Related Cataract.Methods: 210 normal controls and 333 age related cataract cases (110 Nuclear cataract ,110 Cortical cataract and 113 Posterior Sub Capsular types) were screened for mutation inthe sequence extending over exons 4 - 5 of IDO gene by SSCP analysis. Mutant samples weresequenced to identify SNP causing mobility shift and were further genotyped by RFLP analysisusing Hha I enzyme.Results: The substitution of G> A (rs4613984) creating a site for Hha I in 4th intron of IDOgene was detected in 6 out of 333 samples of which one was homozygous Nuclear and 5were heterozygous(2 nuclear and 3 posteror sub capsular). None of the 210 controls showedthe variation. The samples are under study for changes in the gene expression caused by themutation.Conclusions: The variant (G>A; rs4613984) in intron 4 of IDO found in cataract cases andin none of the controls suggests the possibility of its causative role in the development of AgeRelated Cataract.