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Basic Poster Sessions79antibiotics with and without Beta-lactam inhibitor was carried out. The results of PCR andconventional method results were compared.Results: PCR-DNA sequencing of 40 Ocular isolates showed presence of TEM gene in11(27.5%). Five among the 11 were TEM-1 and 6 strains were TEM-116. Eleven others (27.5%)were positive for type 1 OXA and 6 more for AmpC gene. Three among the 11 belonged toAmpC-6 and 3 AmpC -8. None were positive for SHV. Only seven out of these 40 isolates werepositive by conventional method.Conclusions: Our data showed that TEM-116, TEM-1 and OXA-1 were the most prevalentESBL’s followed by AmpC type 8, 6 among ocular isolates. Molecular method showed increasedpositivity for the detection of ESBL’s than conventional method.IBP 031Association Between the Indel Variant in the LOC387715/ARMS2 Gene and AgerelatedMacular Degeneration in South Indian populationSrikrupa Natarajan, 1 Manoharan Aarthi, Ronnie George, 2 Manmath Kumar Das, 3 V L Ramprasad, GKumaramanickavel, Lingam Vijaya, 2 S Sripriya, 1 Parveen Sen 31Department of Genetics and Molecular Biology, Vision Research Foundation, SankaraNethralaya, Chennai, India, 2 Department of Glaucoma, 3 Department of Vitreo Retinal Clinic,Medical Research Foundation,Sankara Netralaya, Chennai, India.Purpose: Age-related macular degeneration (AMD) is a slow progressive disease and aleading cause of visual degeneration in developed countries. Several environmental / geneticrisk factors predisposing to AMD were identified. ARMS is one of the probable candidate geneidentified through genome wide scan at the chromosome locus 10q26. In the current study, theindel variant in 3’UTR of the ARMS2 gene was screened for its possible association with AMDin South Indian population.Methods: Genomic DNA samples from a cohort of 251 individuals from south India wereobtained [134 cases and 117 age-matched controls]. The region in the 3’UTR containing the*372_815del443ins54 variant was PCR amplified and sequenced using ABI 3100 Avant GeneticAnalyzer. Chi square test was performed for statistical analysis.Results: In the current study, the indel variant (*372_815del443ins54) has been observed ata frequency of 64% in cases against controls (36%). A highly significant association betweenthe indel variant and AMD (p=1.5x10-10, odds ratio (OR) = 8.42, 95%confidence interval (CI)–3.96 -17.91) was observed in the current study.Conclusions: ARMS2 has a key role in AMD, pathology possibly through mitochondria-relatedpathways. The indel variant that removes the poly(A) signal sequence and inserts a AU-richelement in the 3’UTR of the ARMS2 gene mediating a rapid mRNA turnover is shown to behighly associated with AMD. However, further studies in a large sample size and functionalanalysis are essential to prove the effect of this polymorphisms in disease pathology.