IERG Abstracrt Book.indd - LV Prasad Eye Institute

66 Basic Poster SessionsAmplex red fluorescent assay with Ex 563nm and Em 587nm. Western blot analysis was doneto detect the presence of LOX and its isoforms.Results: The mean specific activity of LOX in plasma was 0.0498 ± 0.01and 0.1002 ± 0.06 nM/min/mg protein in PXF and control cases respectively while the aqueous humour showed 5.57± 1.5 and 9.79 ± 3.7µM/min/mg protein. Western blot analysis showed the presence of LOXand its isoforms L1 and L2 in both plasma and aqueous.Conclusions: This study shows the presence of LOX protein and its isoforms L1 and L2 bothin plasma and aqueous of cataract and PXF cases.IBP 010Detection of CMV Retinitis in HIV Infected Individuals: A Comparative StudyJambulingam Malathi , Murali Ishwarya, Yesupadam Samson Moses, Hajib Narahari MadhavanL and T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya,Chennai, India.Purpose: Human Cytomegalovirus causes significant morbidity and mortality inimmunocompromised individuals namely transplant recipients and HIV infected individuals.CMV retinitis is the most common form of infection in HIV infected individuals. The aim of thepresent study is to determine the most appropriate specimens for diagnosis of CMV retinitisin HIV patients by testing their blood and aqueous humor or vitreous humor.Methods: As a preliminary step to this study the aqueous humor and blood of 12 HIV infectedindividuals were tested for HCMV genome by real time PCR and by qualitative PCR.Results: Out of the 12 individuals, nine of them were positive for the HCMV genome fromblood while all twelve of them were showed the presence of HCMV in their aqueous humor.From the results of the study we infer that aqueous humor is a better choice for the detectionof CMV retinitis than blood.Conclusions: Since the study group is very small further investigation is required in this fieldto understand the better mode of detection of HCMV.IBP 011Ryanodine Receptor in Lipid Raft Microdomains are Affected by PharmocologicalReagents which Perturb Calcium Dynamics in Muller Glia of RetinaK Gopinath, Nivedita ChatterjeeDepartment of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai,India.Purpose: We have examined the existence of proteins involved in the metabotropic Ca2+signaling cascade as macromolecular aggregates in membrane microdomains in isolated primarybovine Muller glial cells.Methods: Detergent-based lipid raft extraction and density gradient centrifugation. Westernblot analysis, calcium imagingResults: Purinergic stimulation of primary bovine Muller glia showed increased intracellularcalcium levels. As evidenced by the changes in calcium release and calcium wave propagation