XMIPP introductory demo

Figure 6: Manual Particle Selection GUI• You can apply filters to the micrographs, so that you may see the particles better. Filtersare added into a queue, so whenever you change the visualized area, they are appliedagain. Select the menu filter in the overview window and add as many filters as you like.You can clean the filter queue, if you want to return to the original image.• By default particle are marked with a small circle. Some people like larger circles (adjustedto the size of their particles) or squares better. You may change this using theFile->Change mark radius and File->Change mark type menu options.• VERY IMPORTANT! Don’t forget to save the coords in BOTH micrographs before youexit. You can save the angles in one of the File menu of the two micrographs indistinctly.For many specimens, in particular for particles embedded in vitrified ice and of relativelysmall size (100-500 kDa), particle selection is a major obstacle for single particle analysis. Forthese cases, automated procedures usually perform much worse than manual picking. Therefore,a careful interactive selection of the particles, although being time-consuming, will generally14