Tour-de-Force

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Tour-de-Force: Interplay between Mitochondria and Cell Cycle Progression Fall 2007Financial Overview and Time LineProductPriceBlotting & Co-IP Kits € 4,822.00Cell Lines € 8,000.00Fractionation Kits € 545.00Mutations & Mutation Kits € 3,615.00Primary Antibodies € 5,838.00Proteins € 2,660.00Real Time PCR € 1,347.00Secondary Antibodies € 382.33siRNA € 1,831.12Staining € 1,322.0010% unexpected costs € 3,000.00Total € 33,362.45Annual Stipend Researcher € 40,000.00Total € 160,000.00Total Cost Research € 193,362.45Year 1 Year 2 Year 3 Year 4Hypothesis 1Hypothesis 2Hypothesis 3Hypothesis 4Hypothesis 5SCI 332 Advanced Molecular Cell Biology Research Proposal 90
Tour-de-Force: Interplay between Mitochondria and Cell Cycle Progression Fall 2007AcknowledgementsFirst of all, we would like to thank dhr. dr. F.A.C. Wiegant and dhr. prof. dr. J. Boonstra for their devotionto this project. They have been of great help to us.Secondly, we greatly appreciated the help of dhr.dr. L.H.K. Defize. He has provided us with great idea’sand advice on the methodology used in this program.Ms. Martha Lewis, of Santa Cruz Biotechnologies, advised us with choosing the antibodies that wereproposed in this project. We want to thank her for that.Dr. Cristian Scheler, of ProteomeFactory has given great advice on the possibilities of protein sequencing,and thus we thank him for that.Ms. Silvia Otternberg, of Thermo Scientific Pierce Protein Research Products, has provided detailedinformation on the use of cell fractionation to which we are thankful.Mr. Rizwan Farooqui, Thermo Scientific Pierce Protein Research Products, for providing information onCo immunoprecipitation ,which was of great help.Dr. Jamil Talhouk , Of Biovision. He provided our research with additional insight on cell fractionation.Lastly, Ms. Belin Chen, from Abnova, has been a great source of information on the usage of a Mfn2 C-terminal antibody. We are very thankful.SCI 332 Advanced Molecular Cell Biology Research Proposal 91
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