organisation - the Instituto Gulbenkian de Ciência

in known piRNA mutants. We combined eggshell ventralisation with BicD antibody
staining into a screen protocol highly biased toward piRNA mutants. We
will rescreen our collection for new piRNA genes. We will also map the candidate
“effector” troya. The troya phenotype suggests susceptibility for DDR activation.
65% of the lines are screened. Six show Bic-D clumps. We validated positives by
staining for H2Av and by qPCR TEs. All lines with clumps show DDR activation
and TE overexpression. One is allelic to rhino another known piRNA gene. Three
singletons are novel piRNA components as they complement all known piRNA
mutantions on the chromosome. troya show clumps and high TE levels when in
trans with any other 2R mutation in the collection. We mapped troya to a region
with two annotated genes.
IGC ANNUAL REPORT ‘11
RESEARCH GROUPS
20