organisation - the Instituto Gulbenkian de Ciência

GENE EXPRESSION
UNIT
Jörg Becker Head
PhD in Biology, University of Bielefeld, Germany, 2001
Research Fellow - Plant Genomics Group
Head of Facility since 2008
The Gene Expression Unit provides DNA microarray services, ranging from experimental
design over complete sample processing to expert advice on data
analysis. We have been an Affymetrix Core Lab with reference status for Gene-
Chip technology in Portugal since 2002. Running a total of 339 microarrays we
have contributed to 32 in-house, national and international projects in 2011
alone.
FACILITY STAFF
Júlia Lobato (Technician)
João Sobral (Technician, started in October)
SERVICES INCLUDE:
• Gene Expression Profiling (3’ IVT, Gene and Exon arrays);
• Genotyping, Cytogenetics (500K array set and SNP 6.0 array);
• Custom array projects;
• RNA and DNA quality analyses (Bioanalzyer);
• Data analysis training (dChip and Chipster).
MAJOR PROJECTS AND ACCOMPLISHMENTS
• Training SNP6 Cytogenetics (5 SNP6 Genotyping arrays for Gene Expression
Unit);
• Sperm Transcriptome (4 Drosophila arrays for Plant Genomics lab);
• NKTreg (5 Mouse arrays (Cellular Immunology lab for IGC associated group
at IMM);
• Myc Factor (1 Medicago arrays for Institute for Plant Genetics, Leibniz University
Hanover, Germany);
• AGP mutant pollen (6 Arabidopsis arrays for Department of Botany, Faculdade
de Ciências da Universidade do Porto, Portugal);
• Cholesterol and Metastasis (6 Human arrays for Centro de Investigação de
Patobiologia Molecular, Lisbon);
• Zinc Finger (3 Human arrays for Faculty of Pharmacy, University of Lisbon);
• Chein Myf5 (2 Mouse Tiling arrays for Patterning and Morphogenesis lab);
• Leukemia (16 human arrays for IGC associated group at IMM);
• Retinoic Acid in Enteric Lymphoid Organogenesis (8 Mouse arrays for IGC
associated group at IMM);
• Drg11 (20 Mouse arrays for Department of Experimental Biology, Faculdade
de Medicina da Universidade do Porto);
• Xspecies (4 Burkholderia arrays for Biological Sciences Research Group,
Instituto Superior Técnico, Lisbon);
• SNP6 Genotyping (3 SNP6 Genotyping arrays for Department of Genetics,
Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisbon);
• Small RNAs (15 Arabidopsis arrays for Oeiras Summer Schools in Evolutionary
Biology, IGC);
• Ontogeny vs. Phylogeny (15 Mouse arrays for Oeiras Summer Schools in
Evolutionary Biology, IGC);
• Regeneration in denervated fins (16 zebrafish arrays for IGC associated
group at IMM);
• Zymosan treatment (12 Anopheles/Plasmodium arrays for UEI Parasitologia
Médica, Instituto de Higiene e Medicina Tropical, Lisbon);
• Stem cell differentiation (2 Human arrays for Department of Medical Genetics,
Faculdade de Medicina da Universidade do Porto);
• Dis delta phenotype (6 Yeast arrays for Control of Gene Expression Lab, ITQB);
• BolA overexpression (6 E.coli arrays for Control of Gene Expression Lab, ITQB);
• NeoSuc clinical trial (34 human arrays for Computational Genomics Group, IGC);
• Cytogenetics (2 Cyto 2.7M arrays for Department of Genetics, Instituto Nacional
de Saúde Dr. Ricardo Jorge, Lisbon);
• Variants (12 Burkholderia arrays for Biological Sciences Research Group,
Instituto Superior Tecnico, Lisbon);
• LMD Myc (5 Medicago arrays for Institute for Plant Genetics, Leibniz University
Hanover, Germany);
B
Fluidics station for staining and washing up to 4 GeneChips in parallel.
Pseudo-coloured image of a GeneChip array after scanning, showing different signal
intensities depending on the expression level of the correspondent transcripts.
IGC ANNUAL REPORT ‘11
FACILITIES AND SERVICES
92