Systems Biology Research topic(s): 1) Medical Systems Biology 2 ...

Systems Biology
Research topic(s):
1) Medical Systems Biology
2) Cancer Stem Cells
3) Advanced genome engineering
Group leader(s):
Prof. Dr. rer. nat. Frank Buchholz
UCC – University Cancer Center
Medizinische Fakultät der TU Dresden
Fetscherstr. 74
01307 Dresden
Ph.: +49-351-210 2888
Fax: +49 -351-210 1289
E-mail: buchholz@mpi-cbg.de
Education:
1985 Abitur, Gymnasium Bördestr. Bremen
1993 Diplom (Biology), University Göttingen, Germany
1998 PhD in Biology, Supervisor: Dr. Francis Stewart, EMBL and University
Heidelberg, Germany
Training:
1994-1998 PhD student,
1998-2002 Postdoctoral fellow with Prof. J.M. Bishop, UCSF, San Francisco, USA
Clinical Experience:
-
Professional Appointments:
2002-2010 Group Leader, MPI-CBG Dresden, Germany
since 2010 full Professor MF-TUD, Dresden, Germany
Publications: More than 50 scientific publications in peer reviewed journals
Funding: Extrabudgetary funding by DFG, BMBF, EU, DKH, Behrens-Weise-
Stiftung
Award: GO-Bio award 2008, Hector-Forschungspreis 2009
Journals: Reviewer for Nature, Science, Nature Methods, Cell Stem Cell and others
Grant review: DFG, Cancer Research UK, Welcome Trust, Swiss Cancer League, KWF
and others
Societies: Member German Society for Stem Cell Research (GSZ)
Group members:
Scientists: Janet Chusainow, Li Ding, Dragomir Krastev, Katharina Ross,
Mirko Theis, Maciej Paszkowski-Rogacz, Anja Nitzsche
PhD students: Debojyoti Chakraborty, Dennis Kappei, Madina Karimova, Jovan
Mircetic, Aylin Camgoz
MD Student: Arne Jahn
Research Technicians: Sebastian Rose, Romy Zschau, Martina Augsburg
Internal collaborators:
Martin Bornhäuser, Gerhard Ehninger, Christian Thiede, Department of Internal Medicine I
Sebastian Brenner, Department of Pediatrics
Claudia Waskow, CRTD
Tony Hyman, MPI-CBG
Francis Stewart, Biotec, TUD
Ingo Röder, Institute for Medical Informatics and Biometry
Stand: 29.03.2011

External collaborators:
Matthias Mann, MPI Munich
Ulrich Hartl, MPI Munich
Andreas Trumpp, DKFZ Heidelberg
Kevin White, University Chicago
Jonathan Weisman, UCSF
Trevor Dale, Cardiff University
Henk Stunnenberg, Njmegen Centre for Molecular Life Sciences
Participation in collaborative research projects (local, national, international):
Etablierung Systembiologie, SMWK
SyBoSS, EU
DiGtoP, BMBF
Oncogenic Networks in AML (DKH)
SPP1356, DFG
SFB655, DFG
Center for Regenerative Therapies (CRTD)
List of ten most important publications 2005–2010:
1. Slabicki, M.M., Theis, M., Krastev, D.B., Samsonov, E.M., Junqueira, M., Paszkowski-
Rogacz, M., Teyra, J., Heninger, A.K., Poser, I., Prieur, F., Truchetto, J., Confavreux, C.,
Marelli, C., Durr, A., Camdessanche, J.P., Brice, A., Shevchenko, A., Pisabarro, M.T.,
Stevanin, G., Buchholz, F. (2010): A genome-scale DNA repair RNAi screen identifies
SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS Biology,
29;8(6):e1000408.
2. Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M.M., Heninger, A.K., Kittler, R.,
Junqueira, M., Shevchenko, A., Schulz, H., Hubner, N., Doss, M.X., Sachinidis, A.,
Hescheler, J., Caldarelli, A., Poser, I., Theis, M., and Buchholz, F. (2009). A genome-scale
RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem
cell identity. Cell Stem Cell, 8;4(5):403-15.
3. Theis, M., Slabicki, M., Junqueira, M., Paszkowski-Rogacz, M., Sontheimer, J., Kittler, R.,
Heninger, A. H., Glatter, T., Kruusmaa, K., Poser, I., Hyman, A. A., Pisabarro, M. T.,
Gstaiger, M., Aebersold, R., Shevchenko, A., and Buchholz, F. (2009) Comparative
Profiling Identifies C13orf3 as a Novel Component of the Ska Complex Required for
Mammalian Cell Division. EMBO J, 28(10):1453-65.
4. Poser, I., Sarov, M., Hutchins, J., Heriche, J-K., Toyoda, Y., Nitzche, A., Pozniakovsky, A.,
Hegemann, B., Bird, A., Pelletier, L., Kittler, R., Naumann, R., Mechtler, K., Durbin, R.,
Stewart, A.F., Peters, J-M., Buchholz, F., and Hyman, A.A. (2008). BAC TransgeneOmics:
A high-throughput method for exploration of protein function in mammals. Nature Methods,
5(5):409-15.
5. Kittler, R., Pelletier, L., Heninger, AK Slabicki, M., Theis, M., Miroslaw, L., Poser, I.,
Grabner, H., Kozak, K., Wagner, J., Surendranath, V., Richter, C., Bowen, W., Habermann,
Stand: 29.03.2011

B., Hyman, A.A., and Buchholz, F. (2007). Genome-wide RNAi profiling of cell cycle
progression in human tissue culture cells. Nature Cell Biology, 9(12):1401-1412.
6. Sarkar, I., Hauber, I., Hauber, J., and Buchholz, F. (2007). HIV-1 Proviral DNA Excision
Using an Evolved Recombinase. Science, 29;316(5833):1912-5.
7. Kittler, R., Surendranath, V., Heninger,A.K., Slabicki, M., Theis, M., Putz, G., Franke,K.,
Caldareli, A., Grabner, H., Kozak, K., Wagner, J., Rees, E., Korn, B., Sachse, C.,
Sönnichsen, B., Guo, J., Schelter, J., Burchard, J., Linsley, P.S. , Jackson, A.L.,
Habermann, B., and Buchholz, F. (2007). Genome-wide resources of endoribonucleaseprepared
short interfering RNAs for specific loss-of-function studies. Nature Methods,
4(4):337-344.
8. Putz, G., Rosner, A., Nuesslein, I., Schmitz, N., and Buchholz, F. (2006). AML1 deletion in
adult mice causes splenomegaly and lymphomas. Oncogene, 25(6):929-39.
9. Kittler, R., Heninger, A. K., Franke, K., Habermann, B., and Buchholz, F. (2005).
Production of endoribonuclease-prepared short interfering RNAs for gene silencing in
mammalian cells. Nat Methods 2, 779-784.
10. Kittler, R., Pelletier, L., Ma, C., Poser, I., Fischer, S., Hyman, A. A., and Buchholz, F.
(2005). RNA interference rescue by bacterial artificial chromosome transgenesis in
mammalian tissue culture cells. Proc Natl Acad Sci U S A 102, 2396-2401.
Stand: 29.03.2011