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Stanton PhD Thesis final_docx - Atrium - University of Guelph

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treatment and weight gain category. Bunk behaviors were observed for the last 65 calves on the<br />

trial, and resting behaviors were observed for the last 44 calves on the trial (Table 3.1). Smaller<br />

sample sizes were based on the availability <strong>of</strong> video tapes with earlier groups not recorded. Bunk<br />

engaged was use to determine if calves stood at the feed bunk but did not consume feed. Bunk<br />

directed behavior was used as an indicator <strong>of</strong> reluctance to place the head through the stanchion,<br />

which is potential inhibitor <strong>of</strong> feed intake. Close and Far Lying were used as measurements <strong>of</strong><br />

affiliation. One group <strong>of</strong> 5 calves was excluded from the study <strong>of</strong> bunk behaviors due to the<br />

failure <strong>of</strong> video recording equipment.<br />

3.2.2 Experimental Study Design<br />

The objective <strong>of</strong> the underlying trial was to identify behavioral and physiological changes<br />

associated with bovine respiratory disease complex (BRD) or administration <strong>of</strong> a long-acting<br />

antibiotic. No calves were diagnosed with BRD in the first week post-weaning. On days 0, 4, 7,<br />

11 and 14 <strong>of</strong> the present study calves were haltered and blood samples were collected via jugular<br />

venipuncture for measures associated with the antibiotic trial.<br />

3.2.3. Statistical Analysis<br />

All statistical analyses were conducted using Statistical Analysis S<strong>of</strong>tware (SAS version<br />

9.1; SAS Institute Inc, Cary, NC, USA).<br />

Means and standard errors for each weight gain category were generated for normally<br />

distributed continuous variables (Proc Means). If the variables were not normally distributed<br />

upon visual assessment, medians were presented with a range for the 1 st and third quartiles (Proc<br />

Means).<br />

68

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