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Practical Gastrointestinal Endoscopy

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18

CHAPTER 2

• Clean all instrument accessories equally scrupulously, including

the air/water and suction valves, water bottles and the cleaning

brushes.

Disinfection

After thorough cleaning:

• attach the manufacturer’s cleaning adapter to the suction,

biopsy and air/water channels;

• flush each channel with detergent fluid ensuring that it emerges

from the distal end of each channel;

• confirm that all air is expelled from the channels. Soak the

instrument in the chosen disinfectant for the recommended

contact time.

Disinfectants

Glutaraldehyde is the most popular agent. It can destroy viruses

and bacteria within 4·min, is non-corrosive (for the endoscopes),

and has a low surface tension, which aids penetration. Most advisory

authorities recommend a 20-minute soak time in glutaraldehyde

for standard situations, assuming adequate precleaning.

More prolonged soaking may be required in cases of known or

suspected mycobacterial disease. The instrument accessories

(valves, water bottles, etc.) are also soaked.

Glutaraldehyde does carry the risk of sensitization, and can

cause severe dermatitis, sinusitis or asthma among exposed

staff. The risk increases with increasing levels and duration of

exposure. Heavy domestic-grade rubber gloves should be worn

when using glutaraldehyde since normal thin medical glove

material is permeable to it, and goggles and/or a face mask can

protect against splashes. Closed system reprocessors and fume

hoods/extraction fans are important. Reprocessors should be

self-disinfecting. The concentration of disinfectant should be

monitored.

Alcohol, peracetic acid, chlorine dioxide, Sterox and other agents

have also been used for endoscope disinfection.

A sterile water supply (special filters may be needed) helps to

reduce the risk of nosocomial infections, such as Pseudomonas.

Rinsing, drying and storing

Following disinfection, reprocessors rinse the instruments internally

and externally to remove all traces of disinfectant, using

the all-channel irrigator. The air, water and suction channels are

perfused with 70% alcohol and dried with forced air before storage.

This must be done manually if reprocessors are not used.

Bacteria multiply in a moist environment, and the importance

of drying instruments after disinfection cannot be overempha-

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