AIDA Image Analyzer for Windows User's Manual - Raytest

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aytest 3 AIDA Basic 37 Data Windows in AIDA Basic. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Image Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Contextual Menu of the Image Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Histogram Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Contextual Menu of the Histogram Window. . . . . . . . . . . . . . . . . . . . . . . . . . 40 Toolboxes of AIDA Basic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Display Control Toolbox. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Transfer Control Pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 Range Control Pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 Gamma Control Pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 Palette Control Pane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 Orientation Control Pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 Publishing Layout Toolbox . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Annotate Control Pane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Protocol Print Control Pane. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Print Options for Page Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Print Options for Image Window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 Print Preview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Export and Import . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Exporting and Importing Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Exporting Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Saving Images as TIFF file . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 iv AIDA Image Analyzer User’s Manual (Windows)
Introduction 1 Overview The AIDA Image Analyzer software is intended for the evaluation and annotation of images that were obtained by digital optical devices such as CCD cameras, flatbed scanners, fluorescence scanners like FLA scanners, or radioluminography scanners like BAS scanners from Fuji. In addition, the software allows the user to load and evaluate images of other types (e.g. TIF, bitmap). AIDA Image Analyzer can utilize any scanning device that offers a TWAIN compatible driver. The drivers for raytest products are included in the software package. The software concept is modular. Besides more general modules like 1D Evaluation or 2D Densitometry, there are specialized modules for high density array evaluation, multi labelling etc. AIDA provides you with specialized modules for all relevant Bio Imaging tasks. Images can be turned, flipped, enlarged, and zoomed. The grayscale representation of the image intensities can be changed to enhance the visibility of the original data. To comply with GLP standards, the original image data are never changed, so you can keep them safely on CD-ROM. All information about the representation of the image and all result data are saved in an extra file that is kept separate from the original image data. 1D application modules allow you to evaluate profiles. Densitometer windows of any width or angle can be drawn through the image. Bands and the direction of the lanes can be detected automatically. The densitometer windows can be used for quantification, the evaluation of RF values or molecular weights, utilizing standards that are present in the same image. AIDA can use calibration standards to convert the relative intensity integrals to absolute measurements (e.g. µg, nmol). The calibration curve can be reviewed and adapted to fit various functions. Molecular masses, DNA sizes, isoelectric points and RF values can be calculated from the migration distance by the use of internal standards. When multiple standard lanes are present, the image distortion (e. g. due to the smiling effect of the gels) is automatically taken into account for the calculation of these parameters. raytest 5
Page 1 and 2: aytest AIDA Image Analyzer for Wind
Page 3: Contents raytest 1 Introduction 5 O
Page 7 and 8: 1 Installing AIDA Image Analyzer 1
Page 9 and 10: 1 Other problems in recognizing the
Page 11 and 12: General Information 2 Program Struc
Page 13 and 14: Data Structure All information proc
Page 15 and 16: originally used to store the scanne
Page 17 and 18: When the user registration is activ
Page 19 and 20: The Full name is used whenever the
Page 21 and 22: Overlays Most measurements on the i
Page 23 and 24: File Menu You use the File menu to
Page 25 and 26: Edit Menu The commands in the Edit
Page 27 and 28: The Images Overlays command is used
Page 29 and 30: With the Autosaving command you can
Page 31 and 32: Window Menu Figure 2-26 Window Menu
Page 33 and 34: The New, Open, Safe, Print and Prin
Page 35 and 36: Evaluation Push Button (Right Pane
Page 37 and 38: AIDA Basic 3 Data Windows in AIDA B
Page 39 and 40: Cut, Copy, Paste and Delete are use
Page 41 and 42: With the Grid command you can lay g
Page 43 and 44: Transfer Control Pane The histogram
Page 45 and 46: Publishing Layout Toolbox The Publi
Page 47 and 48: Here the text can be entered in the
Page 49 and 50: Print Options for Image Window Sele
Page 51 and 52: Click the checkboxes for the variou
Page 53 and 54: Besides the standard features, the
Page 55 and 56:
4 In the Image Export Options dialo
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