- Page 1: Mass Spectrometry D. Figeys The Ott
- Page 5 and 6: Pumping system Sample introduction
- Page 7 and 8: Why is a vacuum necessary? The mean
- Page 9 and 10: Various ionisation methods �� E
- Page 11 and 12: Electrospray ionization 11/21/2005
- Page 13 and 14: Droplet shrinks due to solvent evap
- Page 15 and 16: Two examples of common ESI oxidatio
- Page 17 and 18: The principal outcome of the electr
- Page 19 and 20: ESI + + + + + Direction of electric
- Page 21 and 22: ES spectrum of Rho protein 100 % 68
- Page 23 and 24: 100 % ES spectrum after deconvoluti
- Page 25 and 26: 22+ 11/21/2005 25 22+
- Page 27 and 28: Matrix assisted laser desorption io
- Page 29 and 30: A standard 100 well UV MALDI sample
- Page 31 and 32: Sample plate 2-3 kV + + + + + + + +
- Page 33 and 34: Why is the matrix so important? �
- Page 35 and 36: Matrix Assisted Laser Desorption/Io
- Page 37 and 38: Pumping system Sample introduction
- Page 39 and 40: -(U + V o cosΩt) r r o + − −
- Page 41 and 42: http://elchem.kaist.ac.kr/vt/chem-e
- Page 43 and 44: What if we place three quadrupoles
- Page 45 and 46: Product ion scan or MS/MS MS/MS gas
- Page 47 and 48: MS Neutral loss scan gas Neutral lo
- Page 49 and 50: Time-of Time of-flight flight (ToF)
- Page 51 and 52: Reflectron ion optics: s3 s2 s1 700
- Page 53 and 54:
Time-Of Time Of-Flight Flight MS 11
- Page 55 and 56:
Ion traps: Hyperbolic 11/21/2005 55
- Page 57 and 58:
Ion Trap. Axial Radial toroidal rin
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Once in the trap 11/21/2005 59
- Page 61 and 62:
Ion traps: Linear 11/21/2005 61
- Page 63 and 64:
Ion traps: Orbital 11/21/2005 63
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Lord of the ion rings: Forging of t
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LTQ-Orbitrap: LTQ Orbitrap: 2 nd ge
- Page 69 and 70:
700 L/s 250 L/s Ion path Q 760 Torr
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11/21/2005 71
- Page 73 and 74:
GR q0 SL Pusher Q1 Puller q2 TOF Li
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Fourier transformed ion cyclotron 1
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Protein identification by mass spec
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Protein identification: Bioinformat
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MALDI-TOF spectrum 11/21/2005 81
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Proteomic applications of mass spec
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Protein identification by mass spec
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MEF2A MGRKKIQITRIMDERNRQVTFTKRKFGLM
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Database Searching WWW Sites: Matri
- Page 91 and 92:
AGAATVKENSSTRVKHCAAGAAWKAGGARERAAKT
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Select: TNSDIVETIR m n R m 1 IR m 2
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The peptide fragments give us seque
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The 9 step strategy for manual inte
- Page 99 and 100:
The 9 step strategy for manual inte
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400 300 200 100 0 Step 2: b 2 and a
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400 300 200 100 0 Step 4: Find y n-
- Page 105 and 106:
400 300 200 100 0 Step 6: Find b-io
- Page 107 and 108:
400 300 200 100 0 Peptide sequence:
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Relative Abundance 100 80 60 40 20
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Relative Intensity TOF Binary data
- Page 113 and 114:
80 40 0 Identification of Proteins
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A) B) C) ∆p Sample introduction:
- Page 117 and 118:
A) B) C) HPLC/ autosampler Sample i