Lentiviral Vectors: design, production, and titration

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Lentiviral Vector System (3 rd generation) 5’LTR Ψ SD RRE cPPT hCMV-P eGFP wPRE SIN3’LTR DU3 R U5 New components: DU3 R U5 Internal Ribosome Entry Site: IRES ∆U3 R U5 ∆U3 R U5 Allows the production of two proteins from one mRNA. A Bicistronic RNA. DU3 R U5 2 nd promoter Allows the production of two mRNAs from one vector. ∆U3 R U5
Lentiviral Vector System (3 rd generation) 5’LTR Ψ SD RRE cPPT hCMV-P eGFP wPRE SIN3’LTR DU3 R U5 New components: DU3 R U5 MCS IRES Multiple Cloning Site for transgenes to be expressed: DU3 R U5 Transgene 2 nd promoter IRES 2 nd promoter ∆U3 R U5 ∆U3 R U5 ∆U3 R U5
Page 1 and 2: Lentiviral Vectors: design, product
Page 3 and 4: Lentiviral Vector System 2. Modific
Page 5 and 6: HIV provirus LTR ψ SD Gag Pro 2 nd
Page 7 and 8: Packaging plasmids 4 th generation?
Page 9: Lentiviral Vector Systems 5’LTR
Page 13 and 14: Lentiviral Vector System (3 rd gene
Page 15 and 16: 1 2 3 4 5 DU3 R U5 DU3 R U5 DU3 R U
Page 17 and 18: Lentiviral Vector System: Gene Supp
Page 19 and 20: Lentiviral Vector System: Gene Supp
Page 21 and 22: Lentiviral Vector System Transgene
Page 23 and 24: Control HEPES - Transfection BES -
Page 25 and 26: Titer Analysis Possibilities: Virus
Page 28 and 29: % fluorescence 70 60 50 40 30 20 10
Page 30 and 31: Replication Competent Lentivirus (R

Lentiviral Vectors: design, production, and titration

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