Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
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<strong>Lentiviral</strong> Vector System (3 rd generation)<br />
DU3 R U5<br />
DU3 R U5<br />
ng/ml medium<br />
200<br />
150<br />
100<br />
50<br />
0<br />
CMV<br />
Intron<br />
LV<br />
NGF<br />
NGF<br />
IRES<br />
IRES<br />
No Intron<br />
Intron<br />
∆U3 R U5<br />
∆U3 R U5<br />
Small peptide<br />
Lig<strong>and</strong> is<br />
Produced <strong>and</strong><br />
Expression is<br />
Enhanced<br />
From vector<br />
Containing<br />
Heterologous<br />
Intron