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Lentiviral Vectors: design, production, and titration

Lentiviral Vectors: design, production, and titration

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<strong>Lentiviral</strong> Vector System (3 rd generation)<br />

DU3 R U5<br />

DU3 R U5<br />

ng/ml medium<br />

200<br />

150<br />

100<br />

50<br />

0<br />

CMV<br />

Intron<br />

LV<br />

NGF<br />

NGF<br />

IRES<br />

IRES<br />

No Intron<br />

Intron<br />

∆U3 R U5<br />

∆U3 R U5<br />

Small peptide<br />

Lig<strong>and</strong> is<br />

Produced <strong>and</strong><br />

Expression is<br />

Enhanced<br />

From vector<br />

Containing<br />

Heterologous<br />

Intron

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