Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
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<strong>Lentiviral</strong> Vector Generations<br />
HIV provirus: provirus<br />
LTR<br />
SD ψ<br />
Gag<br />
1 st Generation:<br />
HIV-1 core proteins<br />
Enzymes <strong>and</strong><br />
Accessory factors<br />
From separate plasmid<br />
And env plasmid<br />
2 nd Generation:<br />
Packaging reduced<br />
gag, pol, tat, rev<br />
And env plasmid<br />
3 rd Generation:<br />
Requirement for tat<br />
Eliminated, rev<br />
Moved to separate<br />
plasmid<br />
Pro<br />
pLV<br />
pMD.G +<br />
pLV<br />
pMD.G<br />
pLV<br />
pMD.G<br />
+<br />
+<br />
Pol<br />
Vif<br />
R<br />
U<br />
Tat<br />
Rev<br />
pRSV-Rev or pLP2<br />
RRE<br />
Env<br />
Nef<br />
LTR<br />
pMDLgpRRE or pLP1
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