Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
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<strong>Lentiviral</strong> Vector System<br />
Transgene Packaging Envelope<br />
5’LTR RREcPPT<br />
hCMV-P<br />
pLV<br />
7,438 bp<br />
pLV<br />
eGFP<br />
WPRE<br />
SIN3’ LTR<br />
pMDLgpRRE<br />
8,895 bp<br />
Poly A<br />
hCMV-P<br />
RRE<br />
GAG<br />
Pol<br />
pRSV-Rev<br />
4,174 bp<br />
Poly A<br />
pMD.G<br />
6,010 bp<br />
pMDLpg.RRE pRSV-Rev pMD.G<br />
CaPO 4<br />
Transfection<br />
RSV<br />
REV<br />
CaPO 4<br />
hCMV-P<br />
Poly A<br />
hβ Globin<br />
IVS2<br />
VSV G<br />
Transfected 293T cells
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