Lentiviral Vectors: design, production, and titration

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Lentiviral Vector System (3 rd generation) 5’LTR Ψ SD RRE cPPT hCMV-P eGFP wPRE SIN3’LTR DU3 R U5 ∆U3 R U5 Self Inactivation No LTR promoter interference Integration into the Host Genome ∆U3 R U5 ∆U3 R U5
HIV provirus LTR ψ SD Gag Pro 2 nd Generation vector DU3 R U5 RSV DU3 R U5 Pol Vif R U Tat Rev Env Lentiviral Vector Systems Requires Tat for production 3rd Generation vector Constitutive promoter RSV or CMV Tat is not required RRE Nef ∆ LTR SIN ∆U3 R U5 SIN ∆U3 R U5
Page 1 and 2: Lentiviral Vectors: design, product
Page 3: Lentiviral Vector System 2. Modific
Page 7 and 8: Packaging plasmids 4 th generation?
Page 9 and 10: Lentiviral Vector Systems 5’LTR
Page 11 and 12: Lentiviral Vector System (3 rd gene
Page 13 and 14: Lentiviral Vector System (3 rd gene
Page 15 and 16: 1 2 3 4 5 DU3 R U5 DU3 R U5 DU3 R U
Page 17 and 18: Lentiviral Vector System: Gene Supp
Page 19 and 20: Lentiviral Vector System: Gene Supp
Page 21 and 22: Lentiviral Vector System Transgene
Page 23 and 24: Control HEPES - Transfection BES -
Page 25 and 26: Titer Analysis Possibilities: Virus
Page 28 and 29: % fluorescence 70 60 50 40 30 20 10
Page 30 and 31: Replication Competent Lentivirus (R

Lentiviral Vectors: design, production, and titration

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