Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
Lentiviral Vectors: design, production, and titration
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HIV provirus<br />
LTR<br />
ψ SD<br />
∆<br />
Gag<br />
Pro<br />
Pol<br />
∆<br />
Vif<br />
R<br />
U<br />
Tat<br />
Rev<br />
Env<br />
RRE<br />
2 nd <strong>and</strong> 3 rd generation viral vectors<br />
1. Viral backbone was stripped to allow room for transgenes<br />
2. Development of the Self-Inactivating (SIN) vector<br />
Nef<br />
∆<br />
LTR
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