Lentiviral Vectors: design, production, and titration

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<strong>Lentiviral</strong> Vector System Transgene Packaging Envelope 5’LTR RREcPPT hCMV-P pLV 7,438 bp pLV eGFP WPRE SIN3’ LTR pMDLgpRRE 8,895 bp Poly A hCMV-P RRE GAG Pol pRSV-Rev 4,174 bp Poly A pMD.G 6,010 bp pMDLpg.RRE pRSV-Rev pMD.G CaPO 4 Transfection RSV REV CaPO 4 hCMV-P Poly A hβ Globin IVS2 VSV G Transfected 293T cells
Control HEPES - Transfection BES - Transfection M1 M1 M1 0.29% 99.18% 99.8%
Page 1 and 2: Lentiviral Vectors: design, product
Page 3 and 4: Lentiviral Vector System 2. Modific
Page 5 and 6: HIV provirus LTR ψ SD Gag Pro 2 nd
Page 7 and 8: Packaging plasmids 4 th generation?
Page 9 and 10: Lentiviral Vector Systems 5’LTR
Page 11 and 12: Lentiviral Vector System (3 rd gene
Page 13 and 14: Lentiviral Vector System (3 rd gene
Page 15 and 16: 1 2 3 4 5 DU3 R U5 DU3 R U5 DU3 R U
Page 17 and 18: Lentiviral Vector System: Gene Supp
Page 19 and 20: Lentiviral Vector System: Gene Supp
Page 21: Lentiviral Vector System Transgene
Page 25 and 26: Titer Analysis Possibilities: Virus
Page 28 and 29: % fluorescence 70 60 50 40 30 20 10
Page 30 and 31: Replication Competent Lentivirus (R

Lentiviral Vectors: design, production, and titration

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