Calcium-Binding Protein Protocols Calcium-Binding Protein Protocols

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Contents xi 26 Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson ........................................... 339 27 Gene Expression in Transfected Cells Kate Hughes, Juha Saarikettu, and Thomas Grundström ........... 355 28 Monitoring the Intracellular Free Ca2+ -Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins Anthony Persechini ........................................................................... 365 29 Studying the Spatial Distribution of Ca2+ -Binding Proteins: How Does it Work for Calmodulin? Katalin Török, Richard Thorogate, and Steven Howell ................ 383 Index ............................................................................................................ 409
Page 1 and 2: Methods in Molecular BiologyTM Biol
Page 3 and 4: M E T H O D S I N M O L E C U L A R
Page 5 and 6: © 2002 Humana Press Inc. 999 River
Page 8 and 9: Preface Calcium plays an important
Page 10 and 11: Contents Dedication ...............
Page 14 and 15: PART I. INTRODUCTION AND REVIEWS CO
Page 16 and 17: Contributors JEAN-RENÉ ALATTIA •
Page 18 and 19: Contributors xvii STEPHEN R. MARTIN
Page 20 and 21: Flow Dialysis 1 III METHODS AND TEC
Page 22 and 23: Flow Dialysis 3 1 Quantitative Anal
Page 24 and 25: Flow Dialysis 5 radioactive ligand
Page 26 and 27: 7 Fig. 3. After assembling the flow
Page 28 and 29: Flow Dialysis 9 6. Take out a washe
Page 30 and 31: Flow Dialysis 11 11. Similarly, add
Page 32 and 33: Flow Dialysis 13 apparatus, 0.42 an
Page 34 and 35: Calcium Binding to Proteins 15 2 Ca
Page 36 and 37: Calcium Binding to Proteins 17 Tabl
Page 38 and 39: Calcium Binding to Proteins 19 K 1-
Page 40 and 41: Calcium Binding to Proteins 21 Fig.
Page 42 and 43: Calcium Binding to Proteins 23 of t
Page 44 and 45: Deconvolution of Calcium-Binding Cu
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Absorption and CD Spectroscopy 43 4
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Absorption and CD Spectroscopy 45 T
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Absorption and CD Spectroscopy 51 F
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Absorption and CD Spectroscopy 53 y
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Absorption and CD Spectroscopy 55 s
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FTIR Spectroscopy of Calcium-Bindin
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Steady-State Fluorescence Spectrosc
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Fluorescence Methods for Ca 2+ Exch
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Surface Plasmon Resonance of CaBP 1
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Differential Scanning Calorimetry 1
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Isothermal Titration Calorimetry 12
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MALLS and Sedimentation Equilibrium
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Solution Scattering 137 12 Small-An
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Solution Scattering 139 small-angle
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Solution Scattering 141 3. Method 3
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Solution Scattering 143 For a compl
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Solution Scattering 145 when electr
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Solution Scattering 147 This P(r) f
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Solution Scattering 149 Fig. 1. Rib
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Solution Scattering 151 Fig. 2. Mod
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Solution Scattering 153 The CaM/MLC
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Solution Scattering 155 structure.
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Solution Scattering 157 4. Neutron
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Solution Scattering 159 17. Seaton,
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Ca 2+ -Binding Proteins Using ESI-M
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Synthetic Calcium-Binding Peptides
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Proteolytic Fragments of Ca 2+ -Bin
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Electron Magnetic Resonance Studies
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Cadmium-113 and Lead-207 NMR Studie
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Calcium-43 NMR 217 18 Calcium-43 NM
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Calcium-43 NMR 219 are different an
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Calcium-43 NMR 221 Fig. 1. The soli
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Calcium-43 NMR 223 it is possible t
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Calcium-43 NMR 225 Fig. 4. Dependen
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Calcium-43 NMR 227 Fig. 6. 43 Ca NM
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Calcium-43 NMR 229 15. Andersson, T
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Multiple Sequence Alignment 231 19
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Multiple Sequence Alignment 233 ens
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Multiple Sequence Alignment 235 whe
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Multiple Sequence Alignment 237 the
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Multiple Sequence Alignment 239 pre
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Multiple Sequence Alignment 241 Tab
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Multiple Sequence Alignment 243 Fig
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Multiple Sequence Alignment 245
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Multiple Sequence Alignment 247 Fig
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Multiple Sequence Alignment 249 pro
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Multiple Sequence Alignment 251 27.
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Multiple Sequence Alignment 253 62.
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Isotope Labeling 255 20 Structure D
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Isotope Labeling 257 pad of folded
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Isotope Labeling 259 3. Methods 3.1
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Isotope Labeling 261 cell growth be
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Isotope Labeling 263 Table 1 Taking
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Isotope Labeling 265 and 15 N label
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Protein Structure Calculation from
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Shape and Dynamics of Calcium-Bindi
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Dipolar Couplings for Structure Ref
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Vector Geometry Mapping 317 24 Vect
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Vector Geometry Mapping 319 The ent
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Vector Geometry Mapping 321 2. Any
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Vector Geometry Mapping 323 Fig. 2.
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S-100 Protein Interacting Drugs 325
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Enzymatic Assays 339 26 Enzymatic A
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Enzymatic Assays 341 by CaM, we des
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Enzymatic Assays 343 2.8. CaM Kinas
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Enzymatic Assays 345 Fig. 2. CaM-de
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Enzymatic Assays 347 5. Add 20 nM N
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Enzymatic Assays 349 10. Wash each
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Enzymatic Assays 351 Fig. 5. Charac
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Enzymatic Assays 353 dation then se
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Gene Expression in Transfected Cell
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Monitoring Ca 2+ -Calmodulin Concen
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Spatial Distribution of Ca 2+ -Bind
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Index 409 Index A Absorption spectr
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Index 411 Citrulline assay, see Cal
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Index 413 Molecular modeling, 147-1
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Index 415 Terbium, see Fluorescence
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Calcium-Binding Protein Protocols Calcium-Binding Protein Protocols

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