Calcium-Binding Protein Protocols Calcium-Binding Protein Protocols

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Flow Dialysis 1 III METHODS AND TECHNIQUES TO STUDY CALCIUM-BINDING PROTEINS
2 Yazawa
Page 1 and 2: Methods in Molecular BiologyTM Biol
Page 3 and 4: M E T H O D S I N M O L E C U L A R
Page 5 and 6: © 2002 Humana Press Inc. 999 River
Page 8 and 9: Preface Calcium plays an important
Page 10 and 11: Contents Dedication ...............
Page 12: Contents xi 26 Enzymatic Assays to
Page 15 and 16: xiv Contents of Companion Volume 15
Page 17 and 18: xvi Contributors LESLIE D. HICKS
Page 19: 20 Dean, Kelsey, and Re
Page 23 and 24: 4 Yazawa Fig. 1. Schematic represen
Page 25 and 26: 6 Yazawa Fig. 2. Shop drawing for t
Page 27 and 28: 8 Yazawa DuPont-NEN and the molar c
Page 29 and 30: 10 Yazawa Fig. 4. Examples of the C
Page 31 and 32: 12 Yazawa 5. Plot the calculated Ca
Page 33 and 34: 14 Yazawa 12. Starovasnik, M. A., D
Page 35 and 36: 16 Fig. 1. Molecular structures and
Page 37 and 38: 18 Linse 7. 0.1 M EDTA. Dissolve 37
Page 39 and 40: 20 Linse iterate the other paramete
Page 41 and 42: 22 Linse tive to small alterations
Page 43 and 44: 24 Linse References 1. Tsien, R. Y.
Page 45 and 46: 26 Haiech and Kilhoffer to use the
Page 47 and 48: 28 Haiech and Kilhoffer where γ is
Page 49 and 50: 30 Haiech and Kilhoffer reporter gr
Page 51 and 52: 32 Haiech and Kilhoffer tein with i
Page 53 and 54: 34 Haiech and Kilhoffer Calmodulin
Page 55 and 56: 36 Haiech and Kilhoffer Fig. 3. Mec
Page 57 and 58: 38 Haiech and Kilhoffer We may sugg
Page 59 and 60: 40 Haiech and Kilhoffer 29. Stewart
Page 61 and 62: 42 Haiech and Kilhoffer 62. Becking
Page 63 and 64: 44 Martin and Bayley Fig. 1. Absorp
Page 65 and 66: 46 Martin and Bayley evaporation. C
Page 67 and 68: 48 Martin and Bayley 4. Set the tem
Page 69 and 70: 50 Martin and Bayley Fig. 2. Near-U
Page 71 and 72:
52 Martin and Bayley discussed by M
Page 73 and 74:
54 Martin and Bayley References 1.
Page 75 and 76:
20 Dean, Kelsey, and Reik
Page 77 and 78:
58 Fabian and Vogel are equipped wi
Page 79 and 80:
60 Fabian and Vogel The penetration
Page 81 and 82:
62 Fabian and Vogel perature, ionic
Page 83 and 84:
64 Fabian and Vogel Fig. 3. IR spec
Page 85 and 86:
66 Fabian and Vogel Fig. 4. Lower t
Page 87 and 88:
68 Fabian and Vogel These correlati
Page 89 and 90:
70 Fabian and Vogel Fig. 5. Amide I
Page 91 and 92:
72 Fabian and Vogel 4. ATR-FTIR spe
Page 93 and 94:
74 Fabian and Vogel 25. Georg, H.,
Page 95 and 96:
76 Weljie and Vogel Fig. 1. Simplif
Page 97 and 98:
78 Weljie and Vogel Fig. 3. Steady-
Page 99 and 100:
80 Weljie and Vogel VIS spectrophot
Page 101 and 102:
82 Weljie and Vogel 4. A series of
Page 103 and 104:
84 Table 1 Advanced Fluorescence Me
Page 105 and 106:
86 Weljie and Vogel References 1. L
Page 107 and 108:
Page 109 and 110:
90 Johnson and Tikunova is removed
Page 111 and 112:
92 Johnson and Tikunova function of
Page 113 and 114:
94 Johnson and Tikunova Fig. 2. Rat
Page 115 and 116:
96 Johnson and Tikunova Fig. 3. Rat
Page 117 and 118:
98 Johnson and Tikunova Fig. 4. Ca
Page 119 and 120:
100 Johnson and Tikunova cence inte
Page 121 and 122:
102 Johnson and Tikunova 6. Johnson
Page 123 and 124:
104 Julenius Fig. 1. Under conditio
Page 125 and 126:
106 Julenius 3. Mix 50 µL of NHS s
Page 127 and 128:
108 Julenius Fig. 3. A typical sens
Page 129 and 130:
110 Julenius (2), is used in the Ia
Page 131 and 132:
Page 133 and 134:
114 Lopez and Makhatadze Fig. 1. Th
Page 135 and 136:
116 Lopez and Makhatadze 6. Buffers
Page 137 and 138:
118 Lopez and Makhatadze The excess
Page 139 and 140:
Page 141 and 142:
122 Lopez and Makhatadze Fig. 1. Is
Page 143 and 144:
124 Lopez and Makhatadze 2.5 mL are
Page 145 and 146:
126 Lopez and Makhatadze pendence o
Page 147 and 148:
128 Hicks et al. equipped with a pu
Page 149 and 150:
130 Hicks et al. Fig. 2. Debye plot
Page 151 and 152:
132 Hicks et al. and 1.0 mg/mL for
Page 153 and 154:
134 Hicks et al. Fig. 3. Sedimentat
Page 155 and 156:
136 Hicks et al. 5. Hayes, D. B. (M
Page 157 and 158:
138 Trewhella and Krueger This chap
Page 159 and 160:
140 Trewhella and Krueger of the sc
Page 161 and 162:
142 Trewhella and Krueger Table 1 C
Page 163 and 164:
144 Trewhella and Krueger beam, or
Page 165 and 166:
146 Trewhella and Krueger At very l
Page 167 and 168:
148 Trewhella and Krueger analytica
Page 169 and 170:
150 Trewhella and Krueger collapsed
Page 171 and 172:
152 Trewhella and Krueger P(r) func
Page 173 and 174:
154 Trewhella and Krueger Fig. 3. S
Page 175 and 176:
156 Trewhella and Krueger concentra
Page 177 and 178:
158 Trewhella and Krueger by a Nati
Page 179 and 180:
Page 181 and 182:
162 Doherty-Kirby and Lajoie metal
Page 183 and 184:
164 Doherty-Kirby and Lajoie two Ca
Page 185 and 186:
166 Doherty-Kirby and Lajoie 7. Pep
Page 187 and 188:
168 Doherty-Kirby and Lajoie Fig. 1
Page 189 and 190:
170 Doherty-Kirby and Lajoie Fig. 2
Page 191 and 192:
172 Doherty-Kirby and Lajoie 5. Alt
Page 193 and 194:
174 Doherty-Kirby and Lajoie phosph
Page 195 and 196:
176 Shaw Fig. 1. Ribbon drawing of
Page 197 and 198:
178 Shaw 2.3. Other 1. Chemicals fo
Page 199 and 200:
180 Shaw 3.3.3. Purification 1. Con
Page 201 and 202:
182 Shaw 10. Hodges, R. S., Semchuk
Page 203 and 204:
184 Brokx and Vogel Table 1 An Over
Page 205 and 206:
186 Brokx and Vogel Fig. 1. UV abso
Page 207 and 208:
188 Brokx and Vogel Fig. 2. 20% SDS
Page 209 and 210:
190 Brokx and Vogel it through an a
Page 211 and 212:
192 Brokx and Vogel 8. Weljie, A. M
Page 213 and 214:
Page 215 and 216:
196 Berliner Fig. 1. Aqueous X-band
Page 217 and 218:
198 Berliner in the Bruker instrume
Page 219 and 220:
200 Berliner Fig. 3. ESR spectra of
Page 221 and 222:
202 Berliner Fig. 5. Low-temperatur
Page 223 and 224:
204 Berliner 14. Musci, G., Reed, G
Page 225 and 226:
206 Clarke and Vogel cal calcium-bi
Page 227 and 228:
208 Clarke and Vogel Fig. 1. 113 Cd
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
214 Clarke and Vogel The linewidth
Page 235 and 236:
Page 237 and 238:
218 Drakenberg sands, of Hertz broa
Page 239 and 240:
220 Drakenberg 1. Bloch equations m
Page 241 and 242:
222 Drakenberg Fig. 2. Measurement
Page 243 and 244:
224 Drakenberg Fig. 3. (A) 43 Ca NM
Page 245 and 246:
226 Drakenberg Fig. 5. 43 Ca NMR sp
Page 247 and 248:
228 Drakenberg NMR at sub-mM concen
Page 249 and 250:
230 Drakenberg 30. Shimizu, T., Hat
Page 251 and 252:
232 Weljie and Heringa Table 1 Amin
Page 253 and 254:
234 Weljie and Heringa Table 2 Webs
Page 255 and 256:
236 Weljie and Heringa diction meth
Page 257 and 258:
238 Weljie and Heringa these databa
Page 259 and 260:
240 Weljie and Heringa lineages. Th
Page 261 and 262:
242 Weljie and Heringa compared, an
Page 263 and 264:
244 Weljie and Heringa sequences as
Page 265 and 266:
246 Weljie and Heringa much larger
Page 267 and 268:
248 Weljie and Heringa ment require
Page 269 and 270:
250 Weljie and Heringa 10. Kawasaki
Page 271 and 272:
252 Weljie and Heringa 44. Thompson
Page 273 and 274:
254 Weljie and Heringa
Page 275 and 276:
256 Li et al. In order for these ex
Page 277 and 278:
258 Li et al. 7. Mineral Mixture (s
Page 279 and 280:
260 Li et al. 3. When the 45% D 2O/
Page 281 and 282:
262 Li et al. sion system works eff
Page 283 and 284:
264 Li et al. reliably produce high
Page 285 and 286:
Page 287 and 288:
268 Mal et al. NMR data, X-PLOR (11
Page 289 and 290:
270 Mal et al. Table 1 Simulated An
Page 291 and 292:
272 Mal et al. 3.1.2.1. AMBIGUOUS D
Page 293 and 294:
274 Mal et al. molecular dynamics a
Page 295 and 296:
276 Mal et al. Assuming a rigid bod
Page 297 and 298:
278 Mal et al. assign (segid A and
Page 299 and 300:
280 Mal et al. References 1. Drenth
Page 301 and 302:
282 Mal et al. 33. Jeener, J., Meie
Page 303 and 304:
Page 305 and 306:
286 Werner et al. Our research has
Page 307 and 308:
288 Fig. 1. (A) T 1, T 2 and the he
Page 309 and 310:
290 Werner et al. tion, using gradi
Page 311 and 312:
292 Werner et al. Fig. 3. (A) Rotat
Page 313 and 314:
294 Werner et al. Fig. 4. (A) Line-
Page 315 and 316:
296 Werner et al. Table 2 Models of
Page 317 and 318:
298 Werner et al. 9. Reinhardt, D.
Page 319 and 320:
300 Werner et al. 39. Press, W. H.,
Page 321 and 322:
302 Boyd et al. utilize residual di
Page 323 and 324:
304 Boyd et al. Fig. 1. (A) The sol
Page 325 and 326:
306 Boyd et al. or significant peak
Page 327 and 328:
308 Boyd et al. Fig. 3. Histogram o
Page 329 and 330:
310 Boyd et al. total and NOE energ
Page 331 and 332:
312 Boyd et al. 2. When studying mu
Page 333 and 334:
314 Boyd et al. 4. Downing, A. K.,
Page 335 and 336:
316 Boyd et al. 35. Schulte-Herbrug
Page 337 and 338:
318 Yap et al. image representation
Page 339 and 340:
320 Yap et al. modified EF-hand is
Page 341 and 342:
322 Table 1 Angle and Distance Outp
Page 343 and 344:
324 Yap et al. 2. Ikura, M. (1995)
Page 345 and 346:
326 Kobayashi that S-100A1 and S-10
Page 347 and 348:
328 Kobayashi 3.2. Coupling of Crom
Page 349 and 350:
330 Kobayashi Fig. 2. Tricine/SDS/P
Page 351 and 352:
332 Kobayashi 0.1% TFA at a flow ra
Page 353 and 354:
334 Kobayashi Both recombinant S-10
Page 355 and 356:
336 Kobayashi Fig. 6. Affinity chro
Page 357 and 358:
Page 359 and 360:
340 Walsh et al. verts CaM from an
Page 361 and 362:
342 Walsh et al. 11. Bovine serum a
Page 363 and 364:
344 Walsh et al. Fig. 1. CaM-depend
Page 365 and 366:
Page 367 and 368:
348 Walsh et al. 3.5. NOS Reduction
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
354 Walsh et al. 3. Cho, M. J., Vag
Page 375 and 376:
356 Hughes et al. The electroporati
Page 377 and 378:
358 Hughes et al. 4. 1 M Na 2CO 3.
Page 379 and 380:
360 Hughes et al. range, repeat ste
Page 381 and 382:
362 Hughes et al. Table 1 Examples
Page 383 and 384:
Page 385 and 386:
366 Persechini of the different CaM
Page 387 and 388:
368 Persechini Fig. 1. Schematic re
Page 389 and 390:
370 Persechini 2. 1 L of Terrific b
Page 391 and 392:
372 Persechini Fig. 4. Titration wi
Page 393 and 394:
374 Persechini Table 1 Parameters f
Page 395 and 396:
376 Persechini Fig. 6. The [Ca 2+ -
Page 397 and 398:
378 Persechini determined if the di
Page 399 and 400:
380 Persechini relatively insensiti
Page 401 and 402:
382 Persechini 18. Chafouleas, J. G
Page 403 and 404:
384 Török et al. actions in the c
Page 405 and 406:
386 Török et al. 3. The reaction
Page 407 and 408:
388 Török et al. Fig. 2. Electros
Page 409 and 410:
390 Török et al. Fig. 3. Peptides
Page 411 and 412:
392 Török et al. Table 1 Peptide
Page 413 and 414:
394 Török et al. Fig. 7. Nanospra
Page 415 and 416:
396 Török et al. Fig. 9. Nanospra
Page 417 and 418:
398 Török et al. Fig. 11. Electro
Page 419 and 420:
400 Török et al. 3 µM FL-calmodu
Page 421 and 422:
402 Török et al. Fig. 14. Localiz
Page 423 and 424:
404 Török et al. Fig. 16. Indicat
Page 425 and 426:
406 Török et al. the significantl
Page 427 and 428:
408 Török et al.
Page 429 and 430:
410 Index substitutes, see Fluoresc
Page 431 and 432:
412 Index Stern-Volmer plot, 78, 81
Page 433 and 434:
414 Index Phenothiazines, see Calmo
Page 435:
METHODS IN MOLECULAR BIOLOGY TM •
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