Antibiotic Classification and Modes of Action - bioMerieux

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Customer Education Antibiotic Classification 1 ml 10 Mueller Hinton 1 ml 7 CFU Quantitative Measure MIC – Broth Dilution Reference Technique 0 0.5 0.5 1 1 2 2 4 4 8 8 16 16 µg/ml µg/ml 35°C - 16 - 20 h 0 0.5 1 2 MIC = 4 8 16 µg/ml • MIC = lowest concentration of antibiotic that inhibits growth (measured visually) • Interpretation of quantitative susceptibility tests is based on: � relationship of the MIC to the achievable concentration of antibiotic in body fluids with the dosage given � For treatment purposes, the dosage of antibiotic given should yield a peak body fluid concentration 3-5 times higher than the MIC or � MIC x 4 = dosage to obtain peak achievable concentration © bioMérieux, Inc., Customer Education March 2008 8
Customer Education Antibiotic Classification Role of Antibiotics What is the role of antibiotics? • To destroy the bacterial population Antibiotics have a bactericidal effect. At which drug concentration is the bacterial population killed? • Minimal Bactericidal Concentration = MBC Bactericidal = kills bacteria © bioMérieux, Inc., Customer Education March 2008 9
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