Accela Pump Hardware Manual

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5 Diagnostics Testing the Pump Proportioning Figure 34. Step gradient profile for testing compositional accuracy Absorbance (mAU) 600 480 360 240 120 0 Step 1 20% Step 2 40% Step 3 60% Step 4 80% 10 20 30 Time (min) 40 50 Step 5 100% Step heights should be within ±1% of the theoretical values. In the step gradient shown in Figure 34, the absorbance value for 100% spiked solvent is 600 mAU (600 mAU – 0 mAU). Therefore, the theoretical values for the 20%, 40%, 60%, and 80% steps are 120, 240, 360, and 480 mAU, respectively. A deviation greater than 6 mAU for any of the steps would be a failing result. If the pump passes both the pulsation and proportioning tests, it is working properly. If the pump fails the pulsation test or the proportioning test, try to restore proper performance by cleaning the proportioning valves as described in “Cleaning the LDA Components without Disassembling the LDA” on page 56. If this fails to restore proper pump performance, disassemble and clean the LDA and replace the primary seals as described in “Replacing the Primary Pistons Seals” on page 67. 48 Accela Pump Hardware Manual Thermo Scientific 60
Determining the Delay Volume of the Pump 5 Diagnostics Determining the Delay Volume of the Pump The gradient delay volume of your pump is an important variable if you are developing gradient methods that you plan to transfer to other instruments. The gradient delay volume of the Accela Pump consists of the wetted components that lie in the flow path between the solvent proportioning valves of the pump and the outlet of the LDA. � To determine the gradient delay volume of the pump 1. Set up your LC system: a. Bypass the autosampler by connecting the pump directly to the detector with tubing of known volume. The volume of the inlet tubing to the Accela PDA Detector is 0.32 μL/inch. (P/N 803260, inlet tubing, insulated, red PEEK, 1/16 in. OD × 0.005 in. ID) b. Prepare a spiked methanol solution as described in “Testing the Pump Proportioning” on page 46. c. Fill one solvent reservoir bottle with 100% methanol. d. Fill a second solvent reservoir bottle with the spiked methanol solution. e. Purge the solvent lines to replace the old solvents with the new solvents. 2. Create a data acquisition method. Use the following detector settings and chromatographic conditions to run and monitor the linear gradient listed in Table 5: • Flow: 200 μL/min • Pressure: approximately 100 bar by restriction • Eluents: A: methanol B: spiked methanol • Detection: UV (254 nm) Table 5. Gradient program for testing gradient delay volume Time (min) %Methanol %Spiked Methanol 0.00 100.0 0.0 0.01 100.0 100.0 3. Equilibrate the system until the baseline stabilizes. To equilibrate the system, depending on the data system you are using, do one of the following: • For Xcalibur, go to step step 4. • For ChromQuest, go to step step 5. Thermo Scientific Accela Pump Hardware Manual 49
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Accela Pump Hardware Manual 60157-9
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Regulatory Compliance Thermo Fisher
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WEEE Compliance This product is req
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CAUTION Symbol CAUTION VORSICHT ATT
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Contents Thermo Scientific Accela P
Page 11:
Baseline Noise at Low Pressures. .
Page 14 and 15:
Preface Special Precautions for the
Page 16 and 17: Preface Solvent Requirement Many ch
Page 19 and 20: Introduction The Accela Pump is a m
Page 21 and 22: The following topics describe the m
Page 23 and 24: Pulse Dampening Assembly 1 Introduc
Page 25 and 26: Status LEDs 1 Introduction Function
Page 27 and 28: Installation This chapter provides
Page 29 and 30: Unpacking and Inspecting the Instru
Page 31 and 32: Making the Initial Back Panel Conne
Page 33 and 34: Connecting the System Interconnect
Page 35 and 36: Connecting the Low-Pressure Solvent
Page 37 and 38: Installing the Super Flangeless Fit
Page 39 and 40: d. Unscrew the swaged fitting and v
Page 41 and 42: Figure 18. Connection between the p
Page 43 and 44: Data System Instrument Configuratio
Page 45 and 46: Figure 20. Thermo Foundation Instru
Page 47 and 48: 3 Data System Instrument Configurat
Page 49 and 50: The Instrument Configuration dialog
Page 51 and 52: 6. Configure the Accela Pump option
Page 53: 3 Data System Instrument Configurat
Page 56 and 57: 4 Preparing the Pump for Operation
Page 58 and 59: 4 Preparing the Pump for Operation
Page 61 and 62: Diagnostics Use the following proce
Page 63 and 64: Using ChromQuest to Monitor the Pum
Page 65: 5 Diagnostics Testing the Pump Prop
Page 69: 5 Diagnostics Determining the Delay
Page 72 and 73: 6 Maintenance Flushing the Piston G
Page 74 and 75: 6 Maintenance Minimizing Pump Pulsa
Page 76 and 77: 6 Maintenance Cleaning the LDA Comp
Page 78 and 79: 6 Maintenance Removing the LDA from
Page 80 and 81: 6 Maintenance Removing the LDA from
Page 82 and 83: 6 Maintenance Disassembling the LDA
Page 84 and 85: 6 Maintenance Disassembling the LDA
Page 86 and 87: 6 Maintenance Replacing the Primary
Page 88 and 89: 6 Maintenance Assembling the Liquid
Page 94 and 95: 6 Maintenance Connecting the LDA to
Page 96 and 97: 6 Maintenance Servicing the Proport
Page 98 and 99: 6 Maintenance Replacing a Fuse Repl
Page 100 and 101: 7 Troubleshooting General System Tr
Page 102 and 103: 7 Troubleshooting Pump Troubleshoot
Page 104 and 105: 7 Troubleshooting Pump Troubleshoot
Page 106 and 107: 7 Troubleshooting Baseline Noise at
Page 108 and 109: 7 Troubleshooting Irreproducible Re
Page 110 and 111: 8 Accessories and Replacement Parts
Page 116 and 117:
8 Accessories and Replacement Parts
Page 119 and 120:
Discontinued Versions of the Accela
Page 121 and 122:
Figure 74. Installing the PEEK film
Page 123:
A Discontinued Versions of the Acce
Page 126 and 127:
Index: E delay volume determining 4
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Index: T steel compression ring 20
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