Partec Reagents & mAb Catalog

List of mouse monoclonal antibodies
sPecificity format order no. Qty isotyPe clone reactivity examPle
cd14 Purified 05-7-414 100 µg IgG1 18D11 Hu
FITC 05-7-414F 1 ml*
PE 05-7-414P 1 ml*
APC 05-7-414A 1 ml*
Purified 05-7-143 100 µg IgG1 MEM-15 Hu
FITC 05-7-143F 1 ml*
PE 05-7-143P 1 ml*
PE-Dy647 05-7-143 PD 647 1 ml*
APC 05-7-143A 1 ml*
comment
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of
mature monocytes, macrophages, and neutrophils, where serves as a multifunctional
lipopolysaccharide receptor; it is also released into the serum both as a secreted and
enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecules in
a reaction catalysed by lipopolysaccharide-binding protein (LBP), an acute phase serum
protein. The soluble sCD14 is able to discriminate slight structural differences between
lipopolysaccharides and is important for neutralisation of serum allochthonous lipopolysaccharides
by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory
and infectious processes.
The 18D11 antibody recognises the CD14 antigen (LPS receptor) expressed strongly
on the surface of monocytes, weakly on the surface of granulocytes, macrophages,
dendritic cells and B cells. On flow cytometry it stains > 90% of human peripheral blood
monocytes. The antibody is LPS neutralising.
The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)linked
membrane glycoprotein expressed on monocytes, macrophages and weakly on
granulocytes; also expressed by most tissue macrophages. The antibody MEM-15 also
reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic
patients.
cd15 FITC 05-7-145F 1 ml* IgM MEM-158 Hu
PE 05-7-145P 1 ml*
comment
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide
determinant (3- fucosyl-N-acetyllactosamine) expressed on several glycolipids,
glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells,
monocytes, macrophages, cells of gastric mucosa, nervous system and various tumour
cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x.
Cells with high surface expression of Le(x) antigen exhibit strong self- aggregation, based
on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in
embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its
isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to
confirm diagnosis of Hodgkins disease.
The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine
(3-FAL) strongly expressed on granulocytes, monocytes, macrophages,
mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
12 *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine
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100
ssc Analysed with CyFlow ®
fitc cd14
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0.1 1 10 100 1000