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Partec Reagents & mAb Catalog

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List of mouse monoclonal antibodies<br />

sPecificity format order no. Qty isotyPe clone reactivity examPle<br />

cd235a FITC 05-7-223F 1 ml* IgG2b HIR2 Hu<br />

PE 05-7-223P 1 ml*<br />

mouse<br />

igG1<br />

isotype<br />

control<br />

mouse<br />

igG2a<br />

isotype<br />

control<br />

comment<br />

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on<br />

erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules<br />

provide the cells with a large mucin-like surface, which minimalises aggregation<br />

between erythrocytes in the circulation. GPA is the carrier of blood group M and N<br />

specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of<br />

Hsa, an Streptococcus adhesin.<br />

The antibody HIR2 recognises N-terminal portion of glycophorin A (and weakly of<br />

glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts,<br />

erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL,<br />

but not on all other cells.<br />

Purified 05-8-385 100 µg lgG1 PPV-06<br />

FITC 05-8-385F 1 ml*<br />

PE 05-8-385P 1 ml*<br />

APC 05-8-385A 1 ml*<br />

comment<br />

This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on<br />

a plant pathogen. The specificity of staining by monoclonal antibodies to target antigens<br />

should be verified by establishing the amount of non-specific antibody binding. In general,<br />

non-reactive immunoglobulin of the same isotype is included as a negative control<br />

for each specific monoclonal antibody used in a particular immunoassay.<br />

Purified 05-8-386 100 µg lgG2a PPV-04<br />

FITC 05-8-386F 1 ml*<br />

PE 05-8-386P 1 ml*<br />

APC 05-8-386A 1 ml*<br />

comment<br />

This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on<br />

a plant pathogen. The specificity of staining by monoclonal antibodies to target antigens<br />

should be verified by establishing the amount of non-specific antibody binding. In general,<br />

non-reactive immunoglobulin of the same isotype is included as a negative control<br />

for each specific monoclonal antibody used in a particular immunoassay.<br />

34 *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine

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