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List of mouse monoclonal antibodies sPecificity format order no. Qty isotyPe clone reactivity examPle cd14 Purified 05-7-414 100 µg IgG1 18D11 Hu FITC 05-7-414F 1 ml* PE 05-7-414P 1 ml* APC 05-7-414A 1 ml* Purified 05-7-143 100 µg IgG1 MEM-15 Hu FITC 05-7-143F 1 ml* PE 05-7-143P 1 ml* PE-Dy647 05-7-143 PD 647 1 ml* APC 05-7-143A 1 ml* comment CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released into the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecules in a reaction catalysed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralisation of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes. The 18D11 antibody recognises the CD14 antigen (LPS receptor) expressed strongly on the surface of monocytes, weakly on the surface of granulocytes, macrophages, dendritic cells and B cells. On flow cytometry it stains > 90% of human peripheral blood monocytes. The antibody is LPS neutralising. The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients. cd15 FITC 05-7-145F 1 ml* IgM MEM-158 Hu PE 05-7-145P 1 ml* comment CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3- fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system and various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self- aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkins disease. The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. 12 *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine 500 400 300 200 100 ssc Analysed with CyFlow ® fitc cd14 0 0.1 1 10 100 1000
sPecificity format order no. Qty isotyPe clone reactivity examPle cd16 FITC 05-7-514F 1 ml* IgG1 3G8 Hu PE 05-7-514P 1 ml* APC 05-7-514A 1 ml* FITC 05-7-506F 1 ml* IgG1 HI16a Hu comment CD16 (FcγRIII) is a 50-80 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms FcγRIII-A and –B. FcγRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with the FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIII-A is associated, moreover, with FcεRI-β subunit. Besides IgG, FcεRI-A can be triggered also by oligomeric IgE. FcγRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in the activation and induction of a proadhesive phenotype. The antibody 3G8 reacts with CD16, a low affinity receptor for aggregated IgG (FcγRIII antigen). CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa; expressed on NK cells, monocytes and macrophages) and CD16b (FcγRIIIB; 48 kDa; mainly expressed on neutrophils). The antibody HI16a reacts with CD16, a low affinity receptor for aggregated IgG (FcγRIII antigen). cd18 FITC 05-7-150F 1 ml* IgG1 MEM-48 Hu PE 05-7-150P 1 ml* APC 05-7-150A 1 ml* comment CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. The antibody MEM-48 recognises an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin β2 subunit; β2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. The antibody induces high-affinity conformation of LFA-1 complex. *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine 500 400 300 200 100 List of mouse monoclonal antibodies ssc Analysed with CyFlow ® Pe cd16 0 0.1 1 10 100 1000 13
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