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List of mouse monoclonal antibodies sPecificity format order no. Qty isotyPe clone reactivity examPle cd25 Purified 05-7-159 100 µg IgG1 MEM-181 Hu FITC 05-7-159F 1 ml* PE 05-7-159P 1 ml* PE-Dy647 05-7-159 PD 647 1 ml* APC 05-7-159A 1 ml* FITC 05-7-509F 1 ml* comment CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanised anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor α chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. The antibody HI25a reacts with CD25 (Interleukin-2 receptor α chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. Antigen density of CD25 is upregulated by the stimulation of PHA, ConA and CD3 antibody. cd27 Purified 05-7-161 100 µg IgG2a LT27 Hu FITC 05-7-161F 1 ml* PE 05-7-161P 1 ml* APC 05-7-161A 1 ml* comment CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. The antibody LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells. 16 *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine
sPecificity format order no. Qty isotyPe clone reactivity examPle cd28 FITC 05-7-427F 1 ml* IgG1 KOLT-2 Hu comment The antibody was submitted to CD28 in the Third International Workshop on Human Leukocyte Differentiation Antigens. The monoclonal antibody is directed against the CD28-antigen, which is weakly expressed on human T cells in the resting period and strongly on human cytotoxic/suppressor T cells (molecular mass 44 kDa). The monoclonal antibody also reacts with 30% of human thymocytes and adult T cell leukaemia. The monoclonal antibody does not react with normal human B lymphocytes, monocytes, granulocytes, platelets, erythrocytes, common acute lymphoblastic leukaemia cells or acute myelocytic leukaemia cells. cd29 Purified 05-7-162 100 µg IgG1 MEM-101A Hu, Ms, Por FITC 05-7-162F 1 ml* PE 05-7-162P 1 ml* APC 05-7-162A 1 ml* comment CD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most β1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of β1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a β1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β. The antibody MEM-101A reacts with CD29 antigen (Integrin beta 1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of haematopoietic and non-haematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). cd30 Purified 05-7-163 100 µg IgG MEM-268 comment The antibody MEM-268 recognises the extracellular part of CD30 (Ki- 1 antigen), a 105 kDa single chain glycoprotein expresses on Hodgkin's and Reed-Stemberg cells; it is also found in Burkitt’s lymphomas, virus-infected T and B lymphocytes; and on normal B und T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor). *for 50 tests Hu: human, Ms: mouse, Pri: primate, Por: porcine, Bo: bovine, Can: canine List of mouse monoclonal antibodies 17
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