saqarTvelos samedicino Jurnali Medical Journal of Georgia

49клеток, нами был использован СМА с 0,25%раствором бикарбоната натрия и 20% НЛС.Выделение очищенного капсульного веществаиз полученной биомассы B.anthracis, производилипо модифицированной методике Афанасьева (2),представленной в схеме.УФ-спектроскопия очищенного капсульноговещества показала отсутствие постороннего белкаи нуклеиновых кислот в составе полученногопрепарата. Намечена дальнейшая работа пополучению и испытанию капсульно-соматическойсыворотки в эксперименте.B.ANTHRACIS mier sxvadasxva sakveb niadagze kafsuliswarmoqmnis SedarebiTi Seswavla da gawmendilikafsulis antigenis miRebam.bubaSvili, s.rigvava, d.gogiaSvili,m.naTiZe, n.varZelaSvili, m.qaruxniSvili,b.lasareiSvili, l.qavTaraZe, l.gubelaZe, s.voronovag.eliavas saxelobis baqteriofagiis, mikrobiologiisa davirusologiis institutijilexis paTogenezSi didi roli eniWeba kafsulas. aRmZvreli, organizmSi SeWrisasxvdeba da mravldeba makrofagur sistemaSi, rac ganpirobebulia kafsulis warmoqmniT.mikrobi gamoyofs Zlier momqmed toqsikur faqtorebs, rac iwvevs leikocitebis, mononuklearulmakrofagalurisistemis ujredebis daSlas. am procesis Sedegad gamoiyofamravali citokini da sxva nivTiereba, romelic gansazRvraven daavadebis gamosavals.kvlevis mizani iyo B.anthracis mier sxvadasxva sakveb niadagze kafsulis warmoqmnis SedarebiTiSeswavla da gawmendili kafsulis antigenis miReba. kvlevis Sedegad kafsulismaqsimaluri raodenobis misaRebad SerCeul iqna optimaluri sakvebi niadagi. kerZod,“Brain-Heart”-s polialenturi niadagi (Semadgenloba: 0,25% natriumis bikarbonati, 20%cxenis normaluri Srati, 40% CO 2).aRniSnul sakveb areze, moSenebuli kafsulaSemcveli baqteriuli biomasidan specialuribioteqnologiis gamoyenebiT, miRebul iqna gawmendili kafsulis antigeni.ultraspeqtroskopiiT Catarebulma gamokvlevebma aCvena, rom miRebuli preparatiqimiurad sufTaa da ar Seicavs gareSe cilebsa da nukleinis mJavebs.COMPERATIVE STUDY OF CAPSULAR PRODUCTION ON VARIOUSNUTRIENT MEDIUM BY B.ANTHRACIS; OBTAINING ANDPURIFICATION CAPSULAR ANTIGENM.Bubashvili, S.Rigvava, D.Gogiashvili, M.Natidze, N.Vardzelashvili,M.Karukhnishvili,B.Lasareishvili, L.Kavtaradze, L.Gubeladze, S.VoronovaCapsular plays a significant role in Anthrax pathogenesis. Penetrating into the organism, a causative agentgets and grows in the macrophage system predisposing capsular production. The microbe secretes powerfultoxic agents causing destruction of leucocytes, particularly mononuclear macrophages. As a result of theseprocesses, a set of cytocins and other substances determining disease outcome are isolated.The goal of our research is to study a capsular production on various nutrient media by B.anthracis and obtaina purified capsular substance.As a result of the study, optimal nutrient medium for obtaining maximum capsular amount, has been selected,particularly Brain-Heart polyvalent medium (composition: 0,25% of natrium bicarbonium,20% of normal horseserum, 40% of CO 2).The purified capsular antigen has been obtained from capsular-containing bacterial biomass on the abovementioned medium using special biotechnology.4. saqarTvelos samedicino Jurnali, 1, 2009 w.