Products at a Glance 2024

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www.pelobiotech.com HUVECs have been used in the cultivation of cardiovascular grafts to study fibrinolytic parameters. The versatility of HUVECs is evident in their application in various fields such as medicine, biology, and endothelial dysfunction research. HUVECs have also been employed in the investigation of signaling pathways, including the lactate/NFkB/IL-8 pathway, which plays a crucial role in tumor metabolism and angiogenesis. HUVECs have been utilized to study the interaction between TRPV4 and KCa2.3 in the context of hypertension treatment. The significance of HUVECs in research is further emphasized by their use in the study of autophagy, apoptosis, and oxidative stress, as well as their role in modeling atherosclerosis and investigating protective mechanisms against injury. GFP-tagged with liposomes and viruses HUVECs offer real-time visualization of subcellular structures, facilitating studies on cellular dynamics and interactions. Conditionally immortalized umbilical cord MSCs are valuable for regenerative medicine research, exploring their potential in tissue engineering and cell-based therapies. Breast We isolate epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, as well as patient-derived xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue. These samples are sourced from various species. The use of epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, patient-derived xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue are diverse and significant in various research and clinical applications. Epithelial cells have been utilized in the separation of breast cancer cells from peripherally circulating blood, particularly through the use of antibodies fixed in microchannels, which enables the isolation of breast cancer cells at an early stage of tumor growth and metastasis. Fibroblasts have been employed in regenerative medicine due to their potential to provide appropriate cross-talk and extracellular matrix (ECM) production to maintain tissue homeostasis and enable repair. Additionally, fibroblasts have been used in the treatment of skin disorders, such as recessive dystrophic epidermolysis bullosa, and have been applied as a biological dressing in wound beds. Furthermore, fibroblasts have been studied for their paracrine anti-fibrotic effects on young and senescent human dermal fibroblasts, indicating their potential in wound repair and regeneration. Breast cancer cells from tumors have been utilized in various studies, including the investigation of cancer-associated fibroblasts (CAFs) inducing epithelial–mesenchymal transition (EMT) of breast cancer cells through paracrine TGF-β signaling, and the promotion of breast cancer cell malignancy via CXCL5 secretion in the tumor microenvironment. Additionally, patient-derived tumor cells, combined with three-dimensional culture technology, have been used to form breast cancer organoids that resemble the in vivo tumor structure. Furthermore, patient-derived xenograft (PDX) cells have been employed in studying the progression of breast cancer through the regulation of epithelial and stromal cell signaling in the TGF-β pathway. Breast cancer-associated fibroblasts from breast tissue have been studied for their role in promoting breast tumorigenesis through the secretion of hepatocyte growth factor and the expansion of cancer-stem like cells. Additionally, engineered human breast tissue models have been used to show that ATR-deficient breast stromal fibroblasts enhance the growth of breast cancer cells. Ovaries We isolate epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and ovarian cancer cells from tumors, in addition to patient-derived xenograft (PDX) cells and ovarian cancer-associated fibroblasts from ovarian tissue. These samples are sourced from various species. Epithelial cells are crucial for studying tissue development and differentiation, while fibroblasts play a pivotal role in extracellular matrix synthesis and tissue remodeling. Endothelial cells contribute to angiogenesis and vascular biology, and smooth muscle cells are integral in understanding vascular and muscular physiology. Isolating ovarian cancer cells from tumors facilitates studies on cancer progression and potential therapeutic targets. PDX cells, derived from patient tumors and engrafted into immunocompromised mice, serve as powerful models for preclinical drug testing, allowing researchers to assess treatment efficacy and tumor response in a more clinically relevant context. Ovarian cancer-associated fibroblasts derived from ovarian tissues contribute to the understanding of tumor microenvironment interactions. Testes and prostate We extract epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and prostate cancer cells from tumors, in addition to patient-derived xenograft (PDX) cells and prostate cancer-associated fibroblasts from prostate tissue. These samples are sourced from various species. Additionally, total RNA from the prostate and testes is also available. Epithelial cells are vital for studying tissue structure and function, often applied in investigations related to epithelial biology, wound healing, and drug response. Fibroblasts have been employed in the regulation of biomechanical properties of human microvascular endothelial cells, indicating their significance in cancer cell interactions and the tumor microenvironment. Fibroblasts have been studied for their potential role in the regulation of the immune and epithelial barrier responses in the prostate. Endothelial cells play 18

www.pelobiotech.com an essential role in vessel homeostasis, and their comprehensive phenotyping using flow cytometry is critical in vascular biology research. Interactions between endothelial cells and electrospun fibers have been studied for engineered vascular replacements, highlighting the importance of endothelial cell orientation along blood flow to increase their ability to resist shear stress. Smooth muscle cells from the testes and prostate have been less commonly studied in the provided references, and their specific uses in research and clinical applications are not as extensively documented. However, their potential role in the physiology and pathology of the testes and prostate may include contributions to the understanding of male reproductive health and disorders related to smooth muscle function in these organs. Prostate cancer cells from tumors, PDX cells, and prostate cancer-associated fibroblasts are invaluable tools for prostate cancer research, aiding in drug development, biomarker discovery, and understanding tumor-stroma interactions. Cells derived from the liver In our extensive cell portfolio, we offer over 900 cells. In addition to hepatocytes, we isolate endothelial cells, epithelial cells, and fibroblasts from various liver regions listed below, we also have Kupffer cells, stellate cells, Hematopoietic stem cells, and non-parenchymal cells. Endothelial cells are pivotal in studies related to angiogenesis, vascular biology, and drug delivery, offering insights into liver-specific vascular responses. Epithelial cells play a crucial role in understanding liver tissue regeneration, differentiation, and responses to injury or disease. Hepatocytes Discover our premium Hepatocytes for Drug Testing: Hepatocytes are the ideal choice for your short- and long-term drug screening and testing needs, offering several key advantages: 1. Comprehensive Testing: Our hepatocytes are extensively tested for vital cytochrome P450 (CYP) enzymes, including CYP1A2, CYP2B6, CYP2C8, CYP2C9, and CYP2C19. We also assess Phase I and Phase II metabolism as well as transporter profiling, ensuring their functionality and reliability for your experiments. 2. Ample Supply: We provide hepatocytes in generous lot sizes, delivering 5-10 million viable cells per vial, ensuring you have a consistent and plentiful source of cells for your research. 3. High Cell Viability: Our hepatocytes consistently achieve over 90% cell viability, guaranteeing robust and dependable results for your experiments. 19