AGf~ICULTURAL RESEARCH, PUSA.
AGf~ICULTURAL RESEARCH, PUSA.
AGf~ICULTURAL RESEARCH, PUSA.
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126 PRACTICAL BACTERIOLOGY<br />
cultures in glucose agar, and for testing the<br />
liquefaction of' gelatin.<br />
(h) Sloped, fo]' "strolce" cultnl'e.-This is often<br />
called a "slope" or "slant, " anfl ensures<br />
a maximum surface of' the medium exposed<br />
to the air. Quantities of 5 C.c. of medium<br />
for an ordinary 6 x {} in. tube arc suJIieient.<br />
When a large number of agar<br />
tubes have to be sloped, special<br />
trays, which allow the tubes to<br />
be laid at the correct angle, arc<br />
useful, and mOl'eover they can<br />
be staeked one upon another so<br />
that very little bench space is<br />
required during solidification.<br />
Frcsh agar slopes aCtel' cooling<br />
contain "waterofeonclcnsation"<br />
at the foot oj' the tube, and<br />
thc tubes should be stored and<br />
handled in the vel1tical position<br />
to prevent thc fluid from flowing<br />
over the sllrfacc of the medium<br />
or entering the cotton - wool<br />
st.opper.<br />
1 oz. screw - capped bottles 1 can<br />
conveniently be substit.uted for<br />
test-tubes. The aluminium cap<br />
should have a waxed lllillboal'cl lIel'1'OllIlO(,I! I'll 1"'''1'''/".<br />
washer. 5 c.c. amounts of the ,
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