AGf~ICULTURAL RESEARCH, PUSA.

3Hi PRACTICAL lJAC'l'EIUOLOGY
bu,cilli me found ill large numbers ill the heal'ti blood
and internal organs, especially ill the spleen, which is
enlarged, soft and diffiuent.
OccwTcncc in Human Lcsions.-Trallsmission of thc
infection to man is from an animal source:
(1) Infection may occur through the skin-e.g. in
persons handliug infeeted animals, Cal'cases
or hides, from shaving brushes, etc. - the
resulting lesion being described as a "malignant
pustule "--i.c. an area of' intense inflammation,
with a central slough and with
surrounding inflammatory oedema. Lymphatic
spl'cad may occur, and even. septicaemia.
(2) Infection may result from inhalation of spores
carried in dust or ii.laments of wool from
infected animals, as in the wool factories
-" wool-sorters' disease." The organisms
settle in the lower part of the trachea or in a
large bl'onchus, and an intense inflammatory
lesion results, with haemol'rhage, oedema,
spread to the thoracic glands, involvement
of the lungs, and effusion into the perieardial
and pleural cavities; the organisms arc present
in considerable numbers in the lesions; a
septicaemic condition may also supervenc.
(3) Infection may occur by the intestine, but this
is l'elatively uncommon in man.
Infect-ion in Animals.-Aniumls may be inl'ectcd from pasture
which has become contaminated with J]. anthracis spores
derived from previous case:; of the disease ill the particular
urea. '!'he disease is al:;o spread by Hrtificial foodstl1l'fse,g.
oil-cake. Biting flies-e.g. StrmwillY8-11my convey the
m-ganism, though in an entirely mechanical way.
Experimental Inocttlation.-Guinea-pigs and whiLe
mice are most susceptible. If a guinea-pig is injected
subcutaneously with pathological material containing
the bacilli, 01' with cultures, the animal J.ies usua.lly
within two days, showing a markecl inflammatory
lesion at the site of inoculation and extensive gel-