AGf~ICULTURAL RESEARCH, PUSA.
AGf~ICULTURAL RESEARCH, PUSA.
AGf~ICULTURAL RESEARCH, PUSA.
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186 PRACTICAL BACTERIOLOGY<br />
The leucocyte count of the l'abbit is subject to great<br />
normal variation.<br />
The chief use of the rabbit lies not so much in<br />
diagnostic work as in its value for experimental<br />
purposes. It is extensively used for the production of<br />
immune seTa, such as agglutinating and haemolytic<br />
sera, which are frequently employed for routine<br />
laboratory diagnosis.<br />
UncleI' Certificate "A" of the Home Otrice the<br />
animal may be inoculated intravenously, intrupcritoneaIly,<br />
subcutaneously 01' by scarification, without<br />
the use of au anaesthetic.<br />
Sc{t)'ijica#on.---'l'he hair is removed from the Hank<br />
of ' the animal by lirst clipping and then shaving, or<br />
by means of the depilating mixture described on<br />
p. 184. 'rhe skin is eleansed with aleohol, whieh is<br />
allowed to evaporatc. A lHlJIlber of parallel I:!cratches<br />
are made with a I:!harp sterile scalpel, just suaieicntly<br />
deep to draw blood. The infective material is rubbed<br />
into the scarified area by means of a platinum loop<br />
or the side of the scalpel. ,{'his method is mainly used<br />
for the propagation of vaccinia. virus.<br />
Intravenous inocttlation is employed when material<br />
has to be introduced directly into the circulation.<br />
The marginal vein of the ear is the most convenient<br />
site. 'rhe rabbit lllay be held by an assistant or placed<br />
in a special box so that only its head protrudes. The<br />
hair over the vein should be dry-shaved with a sharp<br />
razor. The vein may be distended fOl' ease of inoculation<br />
either by vigorous rubbing with a piece of cottonwool<br />
or by holding the ea.r over an electric-light globe,<br />
when the heat causes a dilatation of the blood vessels.<br />
According to the amount of material to be injected,<br />
a suitable syringe furnished with a needle is selected<br />
and sterilised by boiling or other means. It is a mistake<br />
to use too fine a needle. The operator faces the animal<br />
and the caris held horillontal hy meam; or the left ha.nd.<br />
The ncedle is kept as IH)udy parallel as possible to<br />
the vein and the point inserted towilrds the head<br />
of the animal. When the injection is completed, the
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