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AGf~ICULTURAL RESEARCH, PUSA.

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8 PRAC'l'ICAL BACTERIOLOGY<br />

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microscope field (vide p. 57). Brownian movement<br />

is generally regarded as due to the impact of the<br />

molecules of the medium in which the organisms are<br />

suspended and is an oscillatory movement within a<br />

limited radius.<br />

Flagella may be "terminal "-i.c. at one or both<br />

ends of the bacterium-and single or mllltiple. They<br />

may be distributed all round the organism, and this<br />

arrangement is described as " peritrichate. "<br />

When there is a single terminal flagellum the term " monotrichate<br />

" is applied; .. amphitricl1ate " indicates thc presence<br />

of a flagellum at each pole; " lophotrichate" refers to the<br />

arrangement of multiple f1agclla at one or both poles.<br />

Among the spirochaet.es motility is generally due<br />

to contractions of the cell body. Though certain<br />

spirochaetes have been described as possessing flagellalike<br />

structures, these organisms are regarded as<br />

characteristically devoid of flagella.<br />

BACTERIAL SroREs.-Smne species develop a highly<br />

resistant resting-phase, 01' spore, by which the individual<br />

survives unfavourable external conditions. The<br />

spore is not a reproductive structure. In the vast<br />

majority of spore-bearing species only one spore<br />

is developed by each vegetative i'm'm. This structure<br />

appears in the cell protoplasm (" endogenous ") and<br />

increases in size, appearing as a round, ovaloI'<br />

elongated body, which may be situated in the centre<br />

of the bacterium (" central "), at the end (" terminal "),<br />

or between the centre and end (" subterminal "). The<br />

relative size of the spore varies with different species.<br />

Spores can withstand all injurious chemical and<br />

physical influences better than the vegetative forms,<br />

and owe certain of their resistant properties to a<br />

dense outer protective membrane. Under favourable<br />

external conditions the membrane ruptures and the<br />

vegetative form is resumed. This process is described<br />

as the " germination " of thc spore.<br />

'l'he spore is not stained by the ordinary methods but<br />

appears as a clear, unstained portion of the bacterial

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