Abstract SCHIRACK, ANDRIANA VAIS. The Effect of Microwave ...

5. Filter through a third sodium sulfate column into a GC vial without insert.
6. Dry to 0.5 mL under nitrogen.
7. Transfer 200 μL to second GC vial with insert, and save remaining 300 μL in
freezer.
8. Blow down sample in insert to 50 μL immediately before starting GC analysis.
GC-O Analysis
1. Use HP 5890 with FID and sniffing port.
2. Both the neutral/basic and acidic fractions are analyzed from every extraction.
3. Two μL are injected using the sandwich technique into a polar capillary column
(DB-WAX) and into a nonpolar column (DB-5). For injection, 2 µL ether, 1 µL air,
and then 2 µL of sample are drawn into a 10 µL injection syringe.
4. Three experienced panelists will sniff the neutral/basic and acidic fractions of
peanut paste extracts on two different columns. The samples will be described
and scored using a 5-point scale.
GC-MS Analysis
1. Use 6890N GC / 5973 MSD and DB-5MS column.
2. Each extract (1 μL) is injected in the splitless mode.
3. Duplicate analyses are performed on each sample.
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