Abstract SCHIRACK, ANDRIANA VAIS. The Effect of Microwave ...

Appendix 2: Quantification of Peanut Volatiles
First, identify the target compounds in the project by GC/MS and GC/FID
based on the retention index, odor property, and mass spectra as compared to
standards. Calculate relative abundance for a general idea of the compound
concentration, and then quantify to find absolute abundance.
exceptions:
To quantify compounds, follow the procedure in Appendix 1, with the following
1. Before beginning, calculate the stock solutions of the compound standards.
Make the highest concentration first, and dilute accordingly to make 5 levels
of standards. Convert all concentrations to a weight per volume basis, using
the compound density. Be sure not to add more than 500 μL or less than
2 μL of the standard to any sample.
2. Make a table to carefully detail the amount of standard added to each
concentration level. Don’t forget the internal standard.
3. Make the stock solutions in methanol, and add these to 100 mL of deodorized
deionized water at the calculated concentrations. All standards can be
extracted at once, unless there are co-eluting peaks.
4. Add 50 mL of ether to each sample, and continue with the procedure in
Appendix 1 from this point on.
5. For GC/MS analysis, inject each of the 5 levels of standard at least 3 times.
Inject each NB fraction on the GC/MS and each acid fraction on the DB-WAX.
Consider using a dedicated syringe for these standards to avoid
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