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Abstract SCHIRACK, ANDRIANA VAIS. The Effect of Microwave ...

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the detector. <strong>The</strong> compounds can then be identified by aroma descriptors in<br />

combination with retention indices and identification by GC-MS. <strong>The</strong> main purpose <strong>of</strong><br />

GC-O is to order the aroma volatiles in a food matrix according to their potential<br />

importance (Ferreira et al., 2002). Although it is possible that a flavor may result<br />

from a single chemical compound, it is more commonly found that the perceived<br />

flavor is a result <strong>of</strong> the interaction <strong>of</strong> several compounds. For example, Bendall<br />

(2001) discovered differences in milk flavor caused by concentration differences in a<br />

set <strong>of</strong> flavor compounds held in common by the two treatments, rather than selective<br />

occurrence <strong>of</strong> compounds uniquely associated with a particular treatment.<br />

<strong>The</strong> volatiles with the most impact on flavor have been identified using a<br />

determination <strong>of</strong> threshold (CHARM or AEDA), measuring the frequency <strong>of</strong> citations<br />

and by assessing intensity (OSME), and by cross modality matching (Ferreira et al.,<br />

2002). Both Charm Analysis and AEDA are based on dilution <strong>of</strong> samples until an<br />

odor is no longer detectable (Drake and Civille, 2002). <strong>The</strong> highest dilution at which<br />

the odor is still detected is converted to a flavor dilution value (FD) in AEDA, or to a<br />

Charm value. <strong>The</strong> charm algorithm gives an estimate <strong>of</strong> sensory intensity apart from<br />

the complexities caused by psychological estimation <strong>of</strong> stimulus intensity (Acree et<br />

al., 1984). However, these techniques require a large number <strong>of</strong> samples and<br />

panelists, making the method time-consuming (Drake and Civille, 2002).<br />

In AEDA (aroma extract dilution analysis), the flavor extract is sequentially<br />

diluted at a certain rate (usually 2-, 3-, 5-, or 10-fold) and each dilution is analyzed<br />

by GC-O by a number <strong>of</strong> judges. A dilution rate <strong>of</strong> 10 was shown to be the best by<br />

simulations although lower dilution rates were advantageous if the compound had a<br />

67

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