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c linica l assessment and lIlanaqelllent wore again dire cted and
co nvinced t o a t tend the nex t c l inic . Care was taken to e nsure
t hat a l l t h e s e assessments were done in t he best po s s i b l e
enviro nmental se tti ng which was qu i et a nd also private.
4.10 : LABORATORY I NVESTIG AT I ONS
Haemoglobi n a na l ys i s t he onl y laborat ory
i nv e s t i g a t i on r nu t i nely car r i e d out on a l l stud y SUb j e c ts. Two
mi llilitre v e no u s b loo d samp l es we r e t a ken from the f ore arms
o f SUbj ects usi ng EDTA v ecuce tnee tubes. Bl ood samp l es were
ob tained on two occasions ; the f irst in t he pre-pa r t um period
whe n s Ubj ects were admitt e d i n l a bour, and t he second during
mo r b i d i t y ll.sseSSGent. All s amples were collecte d f rom study
cent ers at t he sallle time once ea ch d ay an d transported t o the
u n i ve r s i t y ot Science and Technology (UST) Hospital laboratory
where analysis ....a s carried out by one technician. All blood
samples wer e analyz ed within 24 hours o f collect i o n .
Haemoglob i n l evels wer e an alyzed by t he cynmothaema­
globin conc e nt r at i on m'ethod ....i th s pect ro phot omet ry. The
haemoglobin s t a nda r d preparation u s ed co ntormed t o 6 .S 3985,
wi t h a concentration o f 14.3 9 pe r dec ilitre . The i nstr ume nt
us ed was spectronic 20 (Baus c h & Lomb) , a nd mea surements we r e
t aken at a ....av elength o f 54 0 nano metr e s .