2006 ที่น้องแอนทำ.pmd - Mahidol University

Mahidol University Abstract of International Publications 2006
No.26
Author(s) : Anuntagool N, Wuthiekanun V, White NJ, Currie BJ, Sermswan RW,
Wongratanacheewin S, Taweechaisupapong S, Chaiyaroj SC, Strisinha S.
Title : Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different
geographic and clinical origins.
Source : American Journal of Tropical Medicine and Hygiene. 74 (3): 348 – 352, 2006 (Mar).
Document Type : Article.
Keywords : Biofilm formation, Melioidosis, Identification, Diagnosis, Virulence, Antigens, Thailand.
Abstract : Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327
Burkholderia pseudomallei isolates by sodium dodecyl sulfate – polyacryl amide gel
electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and
B) possessing different ladder profiles and a rough LPS without ladder appearances
were identified. All three LPS types were antigenically distinct by immunoblotting.
The predominant type A (97%) produced the lowest amount of biofilm. The two less
common types (smooth type B and rough type) were found more in clinical than
environmental isolates and more in Australian isolates than Thai isolates. These
isolates were more often associated with relapse than with primary infection.
No.27
Author(s) : Anuracpreeda P, Wanichanon C, Chaithirayanon K, Preyavichyapugdee N,
Sobhon P.
Title : Distribution of 28.5 kDa antigen in the tegument of adult Fasciola gigantica.
Source : Acta Tropica. 100 (1 – 2): 31 – 40, 2006 (Nov).
Document Type : Article.
Keywords : Fasciola gigantica, Tegument, 28.5 kDa antigen, G(2) tegumental granule,
Ultrastructure, Monoclonal antibody, Immunoelectron microscopy.
Abstract : Monoclonal antibody (MoAb) specific to 28.5 kDa tegumental antigen (TA) was used
to localize this antigen in various tissues of adult Fasciola gigantica by means of
indirect immunofluorescence, immunoperoxidase and immunogold techniques. The
indirect immunofluorescence and immunoperoxidase detections revealed that this
antigen was concentrated in the tegument particularly in its outer rim, tegumental
cells and their processes, epithelial linings of the oral sucker and the proximal part of
digestive tract. It was also detected at a moderate concentration in spermatogenic
cells in the testes, cells of Mehlis’ gland, oocytes within the ovary, and ovum within
the egg of adult parasites. At TEM level, the immunogold detection showed deposit of
gold particles specifically in G, tegumental granules and on the surface membrane.
Thus, this antigen is expressed in the tegument and associated structures of adult
parasites. And it could be a major component of the G(2) granules which are shown
to fuse with the surface membrane and contribute material to replace the casted – off
membrane. This process is a part of membrane turnover that prevents the parasite
from being attacked by the host immune effector cells.
No.28
Author(s) : Anurukvorakun O, Suntornsuk W, Suntornsuk L.
Title : Factorial design applied to a non – aqueous capillary electrophoresis method for the
separation of beta – agonists.
Source : Journal of Chromatography A. 1134 (1 – 2): 326 – 332, 2006 (Nov).
Document Type : Article.
Keywords : Factorial design, Beta – agonists, Non – aqueous capillary electrophoresis.
Abstract : The aim of this work was to study the effects of both chemical and instrumental
parameters on the separation of beta – agonists (clenbuterol (CLE), salbutamol (SAL)
and terbutaline (TER)) by non – aqueous capillary electrophoresis (NACE) method.
Due to the number of parameters involved and their interactions, factorial experimental
designs (EDs) at two levels was applied to investigate the influence of experimental
factors (ionic strength of the background electrolyte (BGE), organic solvent, injection
time, voltage and temperature) in sets of several CE responses (resolution, (R – S),
number of theoretical plate (N), tailing factor (TF) and migration time (t(m))). As a
compromise between the four responses, the optimum condition was obtained in 18
mM ammonium acetate in methanol (McOH):acetonitrile (ACN):glacial acetic acid
(66:33: 1%, v/v/v) using an injection time of 4 s, the voltage and the temperature of 28
kV and 24 degrees C, respectively. The proposed NACE permitted the baseline
separation of the three beta – agonists within 10.5 min with good repeatability (%RSD
< 3.5%) and linearity (r(2) > 0.99). The developed method was applicable for the
analysis of the beta – agonists in syrup and tablets and the NACE condition was
compatible with a mass spectrometer detector.
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