2006 ที่น้องแอนทำ.pmd - Mahidol University

Mahidol University Abstract of International Publications 2006
No.135
Author(s) : Chaisawang M, Suphantharika M.
Title : Pasting and rheological properties of native and anionic tapioca starches as modified
by guar gum and xanthan gum.
Source : Food Hydrocolloids. 20 (5): 641 – 649, 2006 (Jul).
Document Type : Article.
Keywords : Tapioca starch, Anionic starch, Gums, Gelatinization, Scanning electron microscopy,
Rheology.
Abstract : Effects of guar and xanthan gums on pasting and rheological properties of native and
anionic tapioca starches were studied by using a Rapid Visco – Analyzer (RVA), a
differential scanning calorimeter (DSC), a scanning electron microscope (SEM), and
a rheometer. Results of RVA measurement indicated that addition of gums increased
peak, breakdown, and final viscosities of native tapioca starch. This effect was more
pronounced for guar gum than xanthan gum. Setback viscosity was increased by
guar gum, but xanthan gum showed the opposite effect. For anionic tapioca starch,
addition of guar gum gave similar results whereas xanthan exhibited opposite results.
Pasting temperatures of all samples with added gums increased in comparison with
the controls which are in good agreement with the increments of the onset (T – o)
and peak (T – p) gelatinization temperatures determined by the DSC. Reduction of
gelatinization enthalpy (Delta H) with gums addition was also observed by the DSC.
SEM micrographs reveal that only xanthan totally wrapped the native starch granules
whereas the other starch – gum mixtures, gums did not wrap the granules.
Measurement of swelling power (SP) demonstrated that the anionic starch exhibited
higher SP than the native starch and addition of gums appeared to increase the SP
values of the mixtures at high temperatures (> 80 degrees C). Results of dynamic
viscoelasticity measurement indicated that addition of gums increased the G’ values
two – fold at all frequencies tested, but the ratio G’’/G’ (tan delta) was decreased. This
suggests that the starch – gum pastes behaved more solid – like characteristics than
the starch alone pastes.
No.136
Author(s) : Chaithirayanon K, Grams R, Vichasri – Grams S, Hofmann A, Korge G, Viyanant V,
Upatham ES, Sobhon P.
Title : Molecular and immunological characterization of encoding gene and 14 – 3 – 3 protein
1 in Fasciola gigantica.
Source : Parasitology. 133: 763 – 775 (Part 6), 2006 (Dec).
Document Type : Article.
Keywords : Fasciola gigantica, 14 – 3 – 3 protein, Tegumental antigens, Expression, Localization.
Abstract : A cDNA encoding Fg14 – 3 – 3 protein 1 was cloned by immunoscreening of an adult
– stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental
antigens of the parasite. The protein has a deduced amino acid sequence of 252
residues and a calculated molecular weight of 28.7 kDa. It shows sequence identity
values between 57.6 and 58.1% to the human 14 – 3 – 3 beta, zeta, theta, and eta
proteins and is in a phylogenetic cluster with the 14 – 3 – 2 protein 1 of Schistosoma
spp. Nucleic acid analyses indicate that the Fg14 – 3 – 3 protein I is encoded by a single
copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult
and 4 – week – old parasites the gene’s transcriptional and translational products
were localized in the gut epithelium, parenchyma, tegument cells, and in the
reproductive organs. An antiserum against recombinant Fg14 – 3 – 3 protein 1 detected
a slightly smaller 14 – 3 – 3 protein in the parasite’s excretion/secretion material and
showed cross – reactivity with 14 – 3 – 3 proteins in extracts of other trematodes and
mouse. Antibodies against Fg14 – 3 – 3 protein were detected in the sera of rabbits as
early as 2 weeks after infection with metacercariae of F. gigantica and the antibody
titre increased continuously over a 10 – week observation period.
No.137
Author(s) : Chaivisuthangkura P, Phattanapaijitkul P, Thammapalerd N, Rukpratanporn S,
Longyant S, Sithigorngul W, Sithigorngul P.
Title : Development of a polyclonal antibody specific to VP19 envelope protein of white spot
syndrome virus (WSSV) using a recombinant protein preparation.
Source : Journal of Virological Methods.133 (2): 180 – 184, 2006 (May).
Document Type : Article.
Keywords : Immunohistochemistry, Penaeus monodon, Polyclonal antibody, VP19, Western blot,
WSSV.
Abstract : The VP19 gene encoding a structural envelope protein of white spot syndrome virus
was cloned into an expression vector and introduced into E. coli. The objective was to
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