2006 ที่น้องแอนทำ.pmd - Mahidol University

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Mahidol University Abstract of International Publications 2006 No.78 Author(s) : Blackman JA, Poulter J. Title : Exact calculations for the ground state properties of the frustrated 2 – dimensional Ising model. Source : Journal of Physics: Conference Series. 30 (1): 139 – 151, 2006 (Feb). Document Type : Conference Paper. Keywords : – Abstract : We have developed a technique that enables the exact calculation of the ground state properties of the 2 – dimensional J Ising model. This paper gives an overview of the methodology and describes the latest results on the correlation function. No.79 Author(s) : Blacksell SD. Title : Laboratory diagnosis of tropical infections. Source : Australian Journal of Medical Science. 27 (1): 3, 2006 (Feb). Document Type : Note. Keywords : – Abstract : – No.80 Author(s) : Blacksell SD. Title : Serological and clinical diagnosis of dengue virus infection – A review of current knowledge. Source : Australian Journal of Medical Science. 27 (1): 26 – 33, 2006 (Feb). Document Type : Review. Keywords : Assay, Dengue virus, Flavivirus, Rapid, Serology. Abstract : Dengue fever, dengue haemorrhagic fever and dengue shock syndrome are tropical diseases that cause significant disease burden. It is estimated that more than 2.5 billion people are at risk of infection and more than 100 countries have endemic dengue virus transmission. This paper reviews the available serological assays for the diagnosis of acute dengue virus infection, differentiation of primary and later infections, and their appropriate application depending on the setting. Dengue clinical syndromes and diagnostic criteria are also described. No.81 Author(s) : Blacksell SD, Doust JA, Newton PN, Peacock SJ, Day NPJ, Dondorp AM. Title : A systematic review and meta – analysis of the diagnostic accuracy of rapid immunochromatographic assays for the detection of dengue virus IgM antibodies during acute infection. Source : Transactions of the Royal Society of Tropical Medicine and Hygiene. 100 (8): 775 – 784, 2006 (Aug). Document Type : Review. Keywords : Dengue, Diagnosis, Immunochromatographic test, IgM, Antibody. Abstract : A meta – analysis of rapid (
Mahidol University Abstract of International Publications 2006 No.82 Author(s) : Blacksell SD, Khounsy S, Phetsouvanh R, Newton PN. Title : A simple and inexpensive container for the transport of biological specimens in limited resource situations. Source : Transactions of the Royal Society of Tropical Medicine and Hygiene. 100 (11): 1084 – 1086, 2006 (Nov). Document Type : Article. Keywords : Biological specimens, Sample transport, Laos. Abstract : We describe a diagnostic specimen transport container that is appropriate for limited resource or emergency settings. The transport container, is constructed from polyvinyl chloride (PVC) plumbing pipe, which is readily available and inexpensive (US$1 – 2, depending on size) and has wide flexibility of size due to the range of PVC pipe dimensions available. The PVC transporters are durable, water – resistant and may be easily decontaminated. They have been adapted for the transport of blood culture bottles from provincial hospitals in Laos, where, during a 2 – year period, 380 PVC tubes containing blood culture bottles were transported without any leakage or breakage. We have found the PVC transporter to be a useful and cost – efficient durable alternative that meets IATA Packing Instruction 650 biological transport container requirements. No.83 Author(s) : Blacksell SD, Newton PN, Bell D, Kelley J, Mammen MP, Vaughn DW, Wuthiekanun V, Sungkakum A, Nisalak A, Day NPJ. Title : The comparative accuracy of 8 commercial rapid immunochromatographic assays for the diagnosis of acute dengue virus infection. Source : Clinical Infectious Diseases. 42 (8): 1127 – 1134, 2006 (Apr). Document Type : Article. Keywords : Linked – immunosorbent assay, Serological Diagnosis, Hemorrhagic – fever, Immunoglobulin – M, Capture ELISA, Antibodies. Abstract : Background: The serological diagnosis of acute dengue virus infection relies on the detection of dengue – specific immunoglobulin M (IgM) antibodies. Immunochromatographic tests are rapid diagnostic tests (RDTs) that can be performed at the bedside, but they have not been fully validated for diagnosis of dengue infection. Methods: More than 20 RDTs for diagnosis of acute dengue infection are commercially available. Of these, 8 were selected for evaluation of performance by use of characterized dengue and nondengue serum specimens, and results were compared with those of a previously published dengue IgM/IgG enzyme – linked immunosorbent assay in conjunction with dengue virus serotyping by reverse – transcriptase polymerase chain reaction. Results: Assay sensitivities were low, ranging from 6.4% (95% confidence interval [CI], 4.0% – 9.7%) to 65.3% (95% CI, 59.9% – 70.5%), and specificities ranged from 69.1% (95% CI, 61.4% – 76.0%) to 100% (95% CI, 97.8% – 100%). Of the 8 tests, only 2 had sensitivities of > 50%, the level considered to be clinically useful, and, of these, 1 had relatively low specificity (69.1%). Samples collected early in the infection were less likely to test positive than those collected later. A thermal stability study demonstrated a loss in performance of some RDTs when they were stored at a high ambient temperature for 3 months. Conclusions: Users of RDTs for dengue should be aware that many of these tests have a diagnostic accuracy that falls well below the manufacturers’ claims. If an acute specimen yields a negative result, a convalescent serum sample should be tested to confirm the result. No RDT adequately differentiated primary and secondary dengue infections, and the tests should not be used for this purpose. No.84 Author(s) : Blessborn D, Neamin G, Bergqvist Y, Lindegardh N. Title : A new approach to evaluate stability of amodiaquine and its metabolite in blood and plasma. Source : Journal of Pharmaceutical and Biomedical Analysis. 41 (1): 207 – 212, 2006 (Apr). Document Type : Article. Keywords : Antimalarial, Amoditiquine, Chloroquine, Desethylamodiaquine, Liquid chromatography, Solid – phase extraction, Stability. Abstract : A stability study for amodiaquine (AQ) and desethylamodiaquine (AQm) in whole blood and plasma is reported. AQ, AQm and chloroquine (CQ) were simultaneously analysed and the ratios AQ/CQ and AQm/CQ were used to ensure correct interpretation of the stability results. CQ was stable in whole blood and plasma at all tested temperatures enabling it to be a stability marker in stability studies. 29
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