2006 ที่น้องแอนทำ.pmd - Mahidol University

Mahidol University Abstract of International Publications 2006
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real – time RT – PCR. It was performed by adding 1/8 of each embryo cDNA to the
PCR mix containing the specific primers to amplify housekeeping gene (beta – actin),
heat shock protein gene (Hsp70), genes related to oxidative stress (MnSOD and
CuSOD), cold stress (CirpB, Rbm3), and cell – cycle arrest (Trp53). We found
upregulation of all six stress – related genes at 3 hr post – warming in pronuclear
stage embryos. Expression of these genes showed much higher level (2 – 33 – fold) in
in – straw vitrification than in in vitro control embryos. In SSV – treated embryos we
could detect only slight changes (0.3 – 2 – fold). At 10 hr post – warming, all genes
were downregulated in embryos vitrified by in – straw method. In SSV – treated
group expression of Hsp70 showed slight increase and Trp53 showed decrease. In
contrast to pronuclear stage, there was no clear pattern of gene expression changes
after vitrification in 8 – cell stage embryos. Several genes were upregulated both at 3
and 10 hr post – warming. Moreover, we found upregulation of beta – actin gene
which we expected to use as a reference gene in in – straw treated embryos in both
3 and 10 hr post – warming, while in pronuclear stage embryos and in SSV treatment
there was no effect on beta – actin expression level. There was no difference in gene
expression between blastocysts developed from fresh or vitrified embryos. In
conclusion, the real – time RT – PCR method from single embryo opened new
opportunities for the understranding of molecular events following cryopreservation.
The upregulation of stress – related genes at 3 hr post – warming in pronuclear stage
embryos might have been an early indicator of reduced viability following in – straw
vitrification in good correlation with the developmental data to blastocyst stage.
No.91
Author(s) : Boonme P, Krauel K, Graf A, Rades T, Junyaprasert VB.
Title : Characterization of microemulsion structures in the pseudoternary phase diagram
of isopropyl palmitate/water/Brij 97: 1 – butanol.
Source : AAPS PharmSciTech. 7 (2): Article No.45, 2006.
Document Type : Article.
Keywords : Microemulsions, Characterization, Colloidal structure, DSC, Cryo – FESEM, NMR.
Abstract : This research was aimed to characterize microemulsion systems of isopropyl palmitate
(IPP), water, and 2:1 Brij 97 and 1 – butanol by different experimental techniques. A
pseudoternary phase diagram was constructed using water titration method. At 45%
wt/wt surfactant system, microemulsions containing various ratios of water and IPP
were prepared and identified by electrical conductivity, viscosity, differential scanning
calorimetry (DSC), cryo – field emission scanning electron microscopy (cryo – FESEM)
and nuclear magnetic resonance (NMR). The results from conductivity and viscosity
suggested a percolation transition from water – in – oil (water/oil) to oil – in – water
(oil/water) microemulsions at 30% wt/wt water. From DSC results, the exothermic
peak of water and the endothermic peak of IPP indicated that the transition of water/
oil to oil/water microemulsions occurred at 30% wt/wt water. Cryo – FESEM
photomicrographs revealed globular structures of microemulsions at higher than
15% wt/wt water. In addition, self – diffusion coefficients determined by NMR reflected
that the diffusability of water increased at higher than 35% wt/wt water, while that of
IPP was in reverse. Therefore, the results from all techniques are in good agreement
and indicate that the water/oil and oil/water transition point occurred in the range of
30% to 35% wt/wt water.
No.92
Author(s) : Boonme P, Krauel K, Graf A, Rades T, Junyaprasert VB.
Title : Characterization of microstructures formed in isopropyl palmitate/water/Aerosol (R)
OT: 1 – butanol (2:1) system.
Source : Pharmazie. 61 (11): 927 – 932, 2006 (Nov).
Document Type : Article.
Keywords : In – oil microemulsions, Transdermal drug – delivery, Resonance energy – transfer,
Mixed surfactants, Diffusion, Behavior, Butanol.
Abstract : The aim of this work was to determine the type and microstructure of microemulsion
samples formed in IPP/water/Aerosol (R) OT: 1 – butanol (2:1) systems as a case study
for the investigation of microemulsions. The concentration of the surfactant/
cosurfactant mixture was kept constant while the ratio of water to oil was varied.
Several techniques were used to investigate the types and phase transitions of the
microemulsion formulations. The experimental methods used included visual
observation cross – polarized light microscopy (PLM) appearance, conductivity,
viscosity, cryo – field emission scanning electron microscopy (cryo – FESEM),
differential scanning calorimetry (DSC), nuclear magnetic resonance (NMR), and