2006 ที่น้องแอนทำ.pmd - Mahidol University

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Mahidol University Abstract of International Publications 2006 No.854 Author(s) : Shimizu T, Kinoshita H, Nihira T. Title : Development of transformation system in Monascus purpureus using an autonomous replication vector with aureobasidin a resistance gene. Source : Biotechnology Letters. 28 (2): 115 – 120, 2006 (Jan). Document Type : Article. Keywords : Aureobasidin A, Autonomous replication vector, Monascus, PKS gene expression. Abstract : To enhance the variety of genetic tools and thus to promote molecular genetic study, aureobasidin A and its resistance gene were adopted as a new marker system together with the incorporation of the Gateway system to facilitate the introduction of long heterologous DNA fragments into Monascus purpureus. The minimum inhibitory concentration of aureobasidin A against Monascus was 0.05 mu g/ml and a transformation efficiency of 17 colonies/mu g DNA was obtained by the protoplast – PEG method with the vector pAUR316, containing the aureobasidin A resistance gene. Southern analysis of the transformants confirmed that pAUR316 exists as an independent vector, demonstrating that the AMA1 sequence acts as the autonomous replication sequence in M. purpureus. Through the use of the Gateway system, a polyketide synthase gene (7.8 kbp) responsible for citrinin biosynthesis was introduced. As a result, the transformants showed 1.5 – fold higher production of citrinin than the wild – type strain No.855 Author(s) : Shiowatana J, Kitthikhun W, Sottimai U, Promchan J, Kunajiraporn K. Title : Dynamic continuous – flow dialysis method to simulate intestinal digestion for in vitro estimation of mineral bioavailability of food Source : Talanta. 68 (3): 549 – 557, 2006 (Jan). Document Type : Article Keywords : In vitro method, Bioavailability, Continuous – flow. Abstract : A system for dynamic continuos – flow dialysis during intestinal digestion for an in vitro Simulation of gastrointestinal digestion is presented as an alternative to human and animal in vivo methods for estimation of the bioavailability of minerals. The method is based on the in vitro batch dialysis method described by Miller, which was developed into a continuous – flow system of a simple design to perform dynamic dialysis in the intestinal digestion stage. A flow dialysis system has the advantages of simulation being close to in vivo physiological conditions because pH change during dialysis is gradual and dialyzed components are continuously removed. The proposed new design performed dialysis during a continuous flow of dialyzing solution (NaHCO 3 ) around a dialysis bag containing peptic digest, which is placed inside a glass dialysis chamber. Gradual change of dialysis pH, similar to that occurring in the gastrointestinal tract, was obtained by optimization of flow rate and concentration of NaHCO 3 . The dialysate collected in fractions was analyzed to determine dialyzed minerals and pH change in the course of dialysis. The method was tested by determination of calcium bioavailability of powder milk and calcium carbonate tablets. No.856 Author(s) : Shiowatana J, Purawatt S, Sottimai U, Taebunpakul S, Siripinyanond A. Title : Enhancement effect study of some organic acids on the calcium availability of vegetables: Application of the dynamic in vitro simulated gastrointestinal digestion method with continuous – flow dialysis Source : Journal of Agricultural and Food Chemistry. 54 (24): 9010 – 9016, 2006 (Nov). Document Type : Article Keywords : Calcium availability, Vegetables, Organic acid, In vitro method, Continuous flow. Abstract : The effect of added organic acids on the calcium availability of vegetables was investigated using the dialysis profiles obtained from an in vitro simulated gastrointestinal digestion with continuous – flow dialysis method. Citric acid was the most effective enhancer followed by tartaric, malic, and ascorbic acids. For amaranth, which has a low calcium availability (5.4%), a significant increase of availability was observed with increasing concentrations of all acids studied. With the continuous – flow dialysis approach, organic acids could be observed to promote the dialyzability even at an elevated intestinal pH. An enhancement effect from added organic acids was not clearly observed for Chinese kale, which itself contains a high amount of available calcium (52.9%). 306
Mahidol University Abstract of International Publications 2006 No.857 Author(s) : Siddhivarn C, Banes A, Champagne C, Riche EL, Weerapradist W, Offenbacher SP. Title : Prostaglandin D – 2 pathway and peroxisome proliferator – activated receptor gamma – 1 expression are induced by mechanical loading in an osteoblastic cell line Source : Journal of Periodontal Research. 41 (2): 92 – 100, 2006 (Apr). Document Type : Article. Keywords : Cyclooxygenase, Mechanical loading, Peroxisome proliferator – activated receptor, Prostaglandin D – 2 synthase. Abstract : Objective: The hypothesis underlying the current study was that the arachidonic acid cascade, specifically activation of the prostaglandin (PG) D – 2 pathway in osteoblasts, is an anabolic signal induced by mechanical loading. Background: Previous studies have shown that mechanical loading of osteoblasts triggers cyclooxygenase (COX) – 2, PGE(2) and prostacyclin (PGI(2)) synthesis. Since modest mechanical loading of osteoblasts promotes bone formation, we sought to determine whether mechanical stress activates the osteoblastic PGD(2) pathway resulting in the synthesis of osteogenic cyclopentenones, including Delta(12)PGJ(2). Methods: Osteoblast monolayers were stretched using a Bioflex apparatus at a frequency of 1 Hz with 1% elongation. Cells and cell media were collected at various time points: 5, 10, 15, 30 min; and 1, 4, 16, 24 h. RNA was extracted for quantitative reverse transcriptase – polymerase chain reaction (RT – PCR). In certain experiments, cells were pre – labeled with C – 14 arachidonic acid prior to stretching. Radiolabeled metabolites in cell media were identified by reverse – phase high performance liquid chromatography (RP – HPLC). Osteoblasts were evaluated for an induction in bone nodule formation by stretching. Results: Mechanical strain significantly increased mRNA expression of COX – 1, COX – 2, PGD(2) synthase and peroxisome proliferator – activated receptor (PPAR) gamma – 1, but not of PPAR gamma – 2 as compared to control unstretched cells (p < 0.05). Mechanical loading stimulated the release of PGE(2), PGD(2) and the PGD(2) metabolite Delta(12)PGJ(2). Mechanical strain resulted in the induction of bone nodules. Conclusions: This report indicates that mechanical loading of osteoblasts results in activation of PGD(2) and the concomitant expression of transcription factor PPAR gamma – 1 mRNA. The coordinated synthesis of Delta(12)PGJ(2), a natural ligand for PPAR gamma – 1, with the increased expression of PPAR gamma – 1, suggests that biomechanical transduction pathways that initially involve the activation of cyclooxygenases may also involve the activation of the Delta(12)PGJ(2) – PPAR pathway. No.858 Author(s) : Sila – Asna M, Bunyaratvej A, Futrakul P, Futrakul N. Title : Letter to the editor: “Renal microvascular abnormality in chronic kidney disease”. Source : Renal Failure. 28 (7): 609 – 610, 2006 (Oct). Document Type : Letter. Keywords : Circulating endothelial cell, Endothelial progenitor cell, Hemodynamic. Abstract : – No.859 Author(s) : Sila – Asna M, Bunyaratvej A, Futrakul P, Futrakul N. Title : Renal microvascular abnormality in chronic kidney disease. Source : Renal Failure. 28 (7): 609 – 610, 2006. Document Type : Letter. Keywords : Circulating endothelial cell, Hemodynamic, Endothelial progenitor cell. Abstract : – No.860 Author(s) : Silpakit C, Sirilerttrakul S, Jirajarus M, Sirisinha T, Sirachainan E, Ratanatharathorn V. Title : The European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ – C30): Validation study of the Thai version Source : Quality of Life Research. 15 (1): 167 – 172, 2006 (Feb). Document Type : Article. Keywords : EORTC QLQ – C30, Fact – G, Quality of Life, Thailand, Validation. Abstract : The objective of this study was to assess the psychometric properties of the Thai version of the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ – C30) version 3.0. The questionnaire was completed by 310 cancer patients during their follow – up at 2 teaching hospital 307
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2006 ที่น้องแอนทำ.pmd - Mahidol University

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