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Baseline study Fish, fry and commercial fishery Nysted Offshore ...

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Bio/consult as Page 85<br />

Figure: 3.10.2. Graphic outline of the procedures in a multivariate distance based test of interaction<br />

between criteria of sectioning. (Legendre <strong>and</strong> Anderson, 1999).<br />

The other method NPMANOVA (Non-parametric multivariate analysis of variance)<br />

(Anderson, 2001) provides a method based on distance-metric of your own choice <strong>and</strong><br />

the product is an analysis of a variance table similar to one from the well-known<br />

analysis of variance. In the variance analytical table, the total sums of squares of<br />

distances are divided into sums of squares belonging to each of the factors of the<br />

analysis such as time, place, <strong>and</strong> the interaction between them. Again, as in the common<br />

ANOVA, these sums of squares are the basis for the F ratios, which again can be tested<br />

for significance using permutations tests. If the distances are calculated as Bray-Curtis<br />

dissimilarities then they also have to be treated in different ways before they can be<br />

used in the NPMANOVA procedure.<br />

It is important to note that both methods can only be used if certain assumptions are<br />

fulfilled just like the requirements to a parametric ANOVA/MANOVA. In principle, the<br />

requirements are the same except that the data do not have to be normal distributed. The<br />

data should still be independent, the effects in the model (time <strong>and</strong> place) should be<br />

additive <strong>and</strong> finally the variation should be uniform between the groups. The<br />

SEAS. <strong>Baseline</strong> <strong>study</strong> – <strong>Fish</strong>, <strong>fry</strong> <strong>and</strong> <strong>commercial</strong> <strong>fishery</strong> Dok. nr. 2148-03-001-rev3 2P.doc

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