student research day - Case Western Reserve University School of ...

Nicholas O. Schroeder
3,4-Methylenedioxy-ß-nitrostyrene (MNS) and the reduction of colony
formation in osteosarcoma cells and osteoblasts.
Nicholas O. Schroeder, Ashley N. Rettew and Edward M. Greenfield
Orthopaedics Department
Case Medical Center
MNS, a known inhibitor of platelet aggregation, has been shown previously to be an inhibitor of tyrosine kinases src and
syk and is being studied as a potential chemotherapeutic agent for osteosarcoma. Our lab has previously shown that MNS
inhibits motility and colony formation in vitro in two families of genetically-related human osteosarcoma cell lines: nontumorigenic/non-metastatic
parental cell lines (TE85 and SAOS-2), a tumorigenic but non-metastatic cell line (MNNG),
and highly tumorigenic/metastatic cell lines (143B and LM7). For this study, we examined the ability of MNS to inhibit
colony formation and disrupt preformed colonies grown on collagen gels to mimic the microenvironment of the lung, the
most common site for lethal micrometastases in osteosarcoma patients. MNS was shown to inhibit colony formation and
quickly induce apoptosis in all five cell lines. Biochemical assays were also performed by an outside party to study the
ability of MNS to inhibit a number of tyrosine kinases believed to be involved in osteosarcoma, including src and syk.
Interestingly, MNS did not inhibit any tyrosine kinases, suggesting that it acts via a different mechanism
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