Inoculum 56(4) - Mycological Society of America

MSA ABSTRACTS
in P. incarnata in experimental pots containing both plants species with field soil.
To identify the mycorrhizal fungi, both plants mycorrhizas were collected from
the experimental pots and compared for restriction fragment length polymorphisms
(RFLP) of the amplified internal transcribed spacer (ITS) region in nuclear
rDNA and ITS sequences. In the experimental pots, significant levels of 13C were
detected from leaves of P. incarnata. Furthermore, the results on PCR-RFLP and
ITS sequences suggested the same species of fungi were colonized on both mycorrhizal
roots of P. incarnata and L. kaempferi that were collected from the experimental
pots. One of those fungi was identified as Thelephora terrestris by ITS
sequence. These results indicate that there are interspecific C transfer from L.
kaempferi to P. incarnata via their common ectomycorrhizal and Pyrola mycorrhizal
hyphal connection. poster
Hatai, Kishio 1 , Muraosa, Yasunori 1 , Morimoto, Kyoko 1 , Sano, Ayaka 2 , Nishimura,
Kazuko 2 and Fujimoto, Kayo 3 . 1 Division of Fish Diseases, Nippon Veterinary
and Animal Science University, Musashino, Tokyo 180-8602, Japan, 2 Research
Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba
260-8673, Japan, 3 Chiba Prefectural Fisheries Research Center, Chiba 295-0024,
Japan. hatai@scan-net.ne.jp. A new fungus isolated from abalone with white
nodules in Japan.
In January 2004, some abalones died from an infection after being imported
from South Africa. Several moribund abalones of about 64 g in body weight
were examined. White nodules were present on the surface of the body parts. A
lower fungus was observed in the nodules under the microscope. To isolate the
fungus, a part of nodule was inoculated onto PYGS agar containing streptomycin
sulfate and ampicillin. After three days on incubation at 15C,a single fungus was
observed growing on the plates that appeared similar to the fungus first observed
under the microscope. The fungus (a strain, NJM 0451) grew well at 15C, but not
at 30C. The fungus was obligate marine fungus, because it only grew on the
PYGS agar. The fungus looked like the fungi of the order Lagenidiales from some
morphological characteristics. Namely, the shape of zoosporangium was like the
middle between the genera Halocrusticida and Haliphthoros. The zoospores were
released from the top of discharge tubes developed from a zoosporangium. The
size of zoospores was bigger than that of the two genera. The phylogenic tree inferred
from 18S rDNA partial sequence using the isolate NJM 0451 and some related
fungi showed that the isolate NJM 0451 was a new genus in the order Lagenidiales.
poster
Hattori, Tsutomu. Forestry and Forest Products Research Institute, Tsukuba 305-
8687, Japan. hattori@affrc.go.jp. Overview of fungal diversity studies in
Japan: toward the establishment of a new RDB.
The Japanese Ministry of the Environment (ME) published the first comprehensive
red data book (RDB) of non-vascular plants, including 91 species of
fungi, in 2000. It was great first step in fungal conservation, but several common
species were involved in the list while some important, rare ones were missed.
ME plans to revise the red list (RL) every 5 years and the RDB every 10 years,
and has organized the Committee for Selection and Evaluation of Threatened
Wildlife of Japan to propose a revised RL in late 2006. For revision of the RL, the
mycology team under the Committee listed macrofungi whose occurrences and/or
known distribution are extremely limited, or whose occurrences are restricted to
environments or hosts that are extremely reduced or undergoing reduction. Mapping
of the listed fungi in addition to those in the last RDB is now in process in
cooperation with amateur mycologists from various areas of the country. Some
other research activities may enhance the scientific values of RLs and RDBs.
Some mycological clubs are recording occurrences of macrofungi in certain sites
over the long-term. Such activities are expected to result in complete species lists
at the local level, while detecting chronosequential reductions of certain species at
the sites. There are already some inventories of host and environmental ranges of
some taxa in certain regions. Such information should be collected for the establishment
of a comprehensive ecological catalog of fungi. Some mycologists are
currently monitoring some rare fungi intensively to detect their distribution, as
well as environments and hosts on which they depend. symposium presentation
Hattori, Tsutomu. Forestry and Forest Products Research Institute, Tsukuba 305-
8687, Japan. hattori@affrc.go.jp. Host recurrence among wood-inhabiting
Aphyllophoraceous fungi in a cool temperate area of Japan.
In order to reveal host recurrences among wood-inhabaiting Aphyllophoraceous
fungi, I established a 200 x 300 m plot at an old growth forest in a cool temperate
area of Japan, and recorded fungi within the plot. I marked all the course
woody debris (CWD) ? 20 cm in diameter within the plot. I recorded DBH or
basal diameter, tree species, and fungal species occurred on each CWD. Decay
class was also recorded for each CWD belonging to three major tree genera in the
forest, Quercus, Castanea and Fagus. To test for host recurrence of the fungi, I
calculated the probability (P) of observing ? the recorded number of a particular
species on the most frequently occurred host tree genus. The following species
were restricted on Quercus and Castanea: Daedalea dickinsii, Hymenochaete rubiginosa,
Melanoporia castanea, Piptoporus soloniensis, Xylobolus frustulatus.
The following species showed high recurrence on Fagus spp. (P