DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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the change in distribution with time. Scale bar is 56.5 µm. Adapted from Tse and
Engler, 2011.
Figure 12| MSCs cultured on 1 and 11 kPa static (top) and gradient (bottom) hydrogels
and stained for B-III- tubulin (red) (neuronal marker) and MyoD (green) (myogenic
marker). Open arrowheads indicate cells expressing either B-III- tubulin or MyoD while
filled arrowheads indicate doubly stained cells. Adapted from Tse and Engler,, 2011.
Figure 13| Quantification of B-III tubulin(grey) and MyoD (black) by MSCs fluorescent
intensity on gradient hydrogels(from 1 to 11 kPa, filled squares) and normalized to the
non-permissive static hydrogels (1 and 11kPa each and only, open circles).Adapted
from Tse and Engler, 2011.
Figure 14| Represent the results of gene (CD73, CD90 and CD105) expression for 7
days (D7) and 14 days (D14) in the Col–HA scaffolds of EDC 0.1% and EDC2.0% which
have stiffnesses of approximately 1and 10 kPa, respectively, and were defined as soft
and stiff substrates, respectively. The results were compared to individual day 1 gene
expression levels. Adapted from Her et al., 2013).
Figure 15| Representative images of phase-contrast microscopy of cells cultured on ≈7
kPa PA hydrogels in which small spots of 2.5 ± 0.5 mm 2 had been previously
functionalized with different COL-1 concentrations (as indicated) to assess cell
adhesion 1 and 6 days after seeding. MSCs were plated at 3000 cells/cm 2 (n=2). Bar
represents 100 µm.
Figure 16| Left and right upper images: Representative fluorescence microscopy
images of hMSCs plated on polyacrylamide hydrogels with 12.5% acrylamide at day 1
(left) and 5 (right) after being fixed and stained with DAPI (in blue). Size bar
corresponds to 200µm. Bottom graphic: Proliferation assay of hMSCs in
polyacrylamide hydrogels, showing the fold increase of the number of cells from day 1
to day 5 (n=3).