DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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xix MSC N n NA Ns NSC NF-H ISCT PA Pa Pax PM qPCR SD TRP TMED TCPs TCP UC UCB UCF UCM WJ V - Mesenchymal stem cell - Nestin - Number of experiments done - Nascent adhesions - Nesprins - Neural stem cell - Neurofilament, Heavy Chain -International Society of Cellular Therapy - Polyacrylamide - Pascal - Paxillin - Proliferation Medium - quantitative reverse transcriptase-polymerase chain reaction - Standard deviation - Transient receptor potential - Tetramethylethylenediamine - Tissue-culture polystyrene - Tissue culture plate - Umbilical Cord - Umbilical cord blood - Umbilical Cord Fragment - Umbilical Cord Matrix - Wharton’s jelly - Poisson ratio
Page 1 and 2: DEPARTAMENTO DE CIÊNCIAS DA VIDA F
Page 3 and 4: ii Queria agradecer à minha namora
Page 5 and 6: iv isolation of hUCM-MSCs was ever
Page 7 and 8: vi Conseguimos optimizar um novo pr
Page 9 and 10: viii Table of contents Acknowledgem
Page 11 and 12: x III. 6 - Influence on MSCs specif
Page 13 and 14: xii Figure 2| Proteins related to t
Page 15 and 16: xiv the change in distribution with
Page 17 and 18: xvi Cells were stained with anti-B-
Page 19: xviii List of abbreviations AA - Ac
Page 24 and 25: 3 I - Introduction I.1 - Mesenchyma
Page 26 and 27: 5 I.1.2.1 - Immunophenotype The Mes
Page 28 and 29: 7 transduced to affect cellular sig
Page 30 and 31: 9 (FXs) and focal adhesions (FA), u
Page 32 and 33: 11 completely understood, they seem
Page 34 and 35: 13 Figure 2 - Proteins related to t
Page 36 and 37: 15 I.2.3 - Mechanotransduction and
Page 38 and 39: 17 2006), such as nestin, an early
Page 40 and 41: 19 Figure 8 - Protein profile depen
Page 42 and 43: 21 It was shown by quantitative rev
Page 44 and 45: 23 and spatially quantify subcellul
Page 46 and 47: 25 Figure 11 - Images of mitomycin
Page 48 and 49: 27 Figure 13 - Quantification of B-
Page 50: 29 I.4 - Objectives It is of intere
Page 54 and 55: 33 II - Materials and methods II.1
Page 56 and 57: 35 al., 2009). Then, the umbilical
Page 58 and 59: 37 Table I - Composition of the hyd
Page 60: 39 Frequency sweeps were performed
Page 64 and 65: 43 III - Results III.1 - Rheologica
Page 66 and 67: 45 Figure 15 - Representative image
Page 68 and 69: 47 III.4 - Isolation and Proliferat
Page 70 and 71:
49 control, we used cells from the
Page 72 and 73:
51 Table III: Mean, Median and Coef
Page 74 and 75:
53 Figure 21- hMSCs cultured on TCP
Page 76 and 77:
Figure 22 - hMSCs cultured on TCP (
Page 79:
58 Chapter IV - Discussion and Conc
Page 82 and 83:
61 The mechanical aspects of the hy
Page 84 and 85:
63 may speculate that FN provides c
Page 86:
65 may have caused the cells to los
Page 90 and 91:
69 III. References Allingham, J.S.,
Page 92 and 93:
71 Eyckmans, J. et al., 2011. A hit
Page 94 and 95:
73 Sanchez-Ramos, J., S. Song, et a
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DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

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