DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

More documents

Recommendations

Info

xv Figure 17| Colonies of hMSCs isolated in different PA hydrogels from UCM-WJ fragments after 1 week and 2 weeks of fragments plating in the hydrogels with just COL-1 or with COL-1 and FN (n=2). Bar corresponds to 200µm. Figure 18| Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is a logarithmic scale of fluorescence. Cells were detached, labelled with antibodies against the indicated antigens and analyzed by flow cytometry. Cells were positive for CD49e, CD73, CD13 and CD90 (red and green lines) when compared with unlabeled MSCs (dark gray and light gray) for the 2 conditions, both from the PA hydrogels and the Plastic/TCPs (n=1). Figure 19| Immunophenotype of UCM-MSCs. The Y axis is the cell density. The X axis is a logarithmic scale of fluorescence. Cells were detached, labelled with antibodies against the indicated antigens and analyzed by flow cytometry. Cells were negative for CD11b, HLA-DR, CD45 (red and green lines) when compared with unlabelled MSCs (dark gray and light gray) for the 2 conditions, both from the hydrogels and of the Plastic/TCPs (n=1). Figure 20| hMSCs cultured on TCPs (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation. Cells were stained with anti-B-III tubulin (red), anti-Nestin antibodies (green) and DAPI (blue). This experiment was performed once (n=1). Bar corresponds to 400µm. Figure 21| hMSCs cultured on TCPs (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation. Cells were stained with anti-GFAP (red), anti-O4 antibodies (green) and DAPI (blue). This experiment was performed once (n=1). Bar corresponds to 400µm. Figure 22| hMSCs cultured on TCP (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation. Cells on the 7kPa hydrogels and 1kPa hydrogels were isolated on 12 kPa hydrogels as mentioned in III.4.
xvi Cells were stained with anti-B-III tubulin (red), anti-Nestin antibodies (green) and DAPI (blue). This experiment was performed once (n=1). Bar corresponds to 200µm. Figure 23| hMSCs cultured on TCP (Plastic) and PA hydrogels coated with COL-1 for 7 days after being treated with mitomycin C to inhibit proliferation, the cells on the 7kPa hydrogels and 1kPa hydrogels were isolated on 12 kPa hydrogels as mentioned in III.4. Cells were stained with anti-GFAP (red), anti-O4 antibodies (green) and DAPI (blue). This experiment was performed once (n=1) for GFAP and twice for O4 (n=2). Bar corresponds to 200µm.
Page 1 and 2: DEPARTAMENTO DE CIÊNCIAS DA VIDA F
Page 3 and 4: ii Queria agradecer à minha namora
Page 5 and 6: iv isolation of hUCM-MSCs was ever
Page 7 and 8: vi Conseguimos optimizar um novo pr
Page 9 and 10: viii Table of contents Acknowledgem
Page 11 and 12: x III. 6 - Influence on MSCs specif
Page 13 and 14: xii Figure 2| Proteins related to t
Page 15: xiv the change in distribution with
Page 19 and 20: xviii List of abbreviations AA - Ac
Page 22: 1 Chapter I Introduction
Page 25 and 26: 4 There are many advantages in usin
Page 27 and 28: 6 only ones MSCs can differentiate
Page 29 and 30: Figure 1. Mechanotransduction in a
Page 31 and 32: 10 1C)(Dogterom et al., 2005). Not
Page 33 and 34: 12 extracellular ligand and the cyt
Page 35 and 36: 14 Figure 3 - The Rigidity Sensing
Page 37 and 38: 16 Figure 5 - Images depicted on A)
Page 39 and 40: 18 Figure 7 - A) Immunofluorescence
Page 41 and 42: 20 (GFAP) (is a class-III intermedi
Page 43 and 44: 22 can specify lineage towards cell
Page 45 and 46: 24 I.3 - Project rationale and expe
Page 47 and 48: 26 Figure 12 - MSCs cultured on 1 a
Page 49 and 50: 28 than that in soft substrate. Thi
Page 52: 31 Chapter II - Materials and Metho
Page 55 and 56: 34 Covance and DakoCytomation, resp
Page 57 and 58: 36 clone SAM1; mouse Pe-Cy7 anti-hu
Page 59 and 60: 38 To functionalize the complete su
Page 62: 41 Chapter III Results
Page 65 and 66: 44 2008) and also as previously est
Page 67 and 68:
12.5% Ac PBS Fold increase of hMSCs
Page 69 and 70:
≈12 kPa / COL-1 +FN ≈12 kPa / C
Page 71 and 72:
50 Figure 19 - Immunophenotype of U
Page 73 and 74:
52 coated with COL-1 (similar to wh
Page 75 and 76:
54 hydrogels of 1 and 7 kPa (for ce
Page 77:
Figure 23 - hMSCs cultured on TCP (
Page 81 and 82:
60 Discussion MSCs are widely used
Page 83 and 84:
62 isolating MSCs on the PA hydroge
Page 85 and 86:
64 2011) and expanded on TCPs for 5
Page 88:
67 Conclusion In conclusion, we opt
Page 91 and 92:
70 Cretu, A., Castagnino, P. & Asso
Page 93 and 94:
72 McBeath, R. et al., 2004. Cell s
Page 95:
74 Wang, N., Butler, J.P., and Ingb
show all

DEPARTAMENTO DE CIÊNCIAS DA VIDA ... - Estudo Geral

Create successful ePaper yourself

Delete template?

Save as template?